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Immunofluorescent staining of human cell line MCF7 shows localization to nucleoli & nuclear membrane.
Antibody dilution
1:76
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA040755
Antibody HPA040868
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 33 tissues Protein: detected in 44 cell types
RNA: detected in 33 tissues Protein: detected in 28 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:500
1:25
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA040755
Antibody HPA040868
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403099)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
86.9, 83.5
86.9, 83.5
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA040755
Antibody HPA040868
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
