ABCB4 - Antibodies - The Human Protein Atlas

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ABCB4

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA049395

Antibody HPA053288

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA049395

HPA053288

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA049395, RRID:AB_2680745

Atlas Antibodies Cat#HPA053288, RRID:AB_2682102

VALIDATION SUMMARY

Antibody HPA049395

Antibody HPA053288

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA049395

Antibody HPA053288

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm, plasma membrane & focal adhesion sites.

Antibody dilution

1:48

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA049395

Antibody HPA053288

Standard validation

Approved

Figure description

Immunohistochemical staining of human liver shows strong positivity in bile canaliculi.

Expression

RNA: detected in 16 tissues
Protein: detected in 15 cell types

RNA: detected in 16 tissues
Protein: detected in 70 cell types

Retrieval

HIER pH6

Antibody dilution

1:50

1:20

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA049395

Antibody HPA053288

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

141.5, 140.7, 135.3

141.5, 140.7, 135.3, 6.9

Antibody dilution

1:240

1:150

PROTEIN ARRAY

Antibody HPA049395

Antibody HPA053288

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:1900

1:1150

ANTIGEN INFORMATION

Antibody HPA049395

Antibody HPA053288

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

HSELMKKEGVYFKLVNMQTSGSQIQSEEFELNDEKAATRMAPNGWKSRLF
RHSTQKNLKNSQMCQKSLDVETDGLEANVPPVS

LEAAKNGTAWRPTSAEGDFELGISSKQKRKKTKTVKMIGVLTLFRYSDWQ
D

Matching transcripts

ABCB4-001 - ENSP00000352135 [100%]
ABCB4-002 - ENSP00000265723 [100%]
ABCB4-009 - ENSP00000392983 [100%]
ABCB4-201 - ENSP00000351172 [100%]

ABCB4-001 - ENSP00000352135 [100%]
ABCB4-002 - ENSP00000265723 [100%]
ABCB4-003 - ENSP00000394511 [100%]
ABCB4-009 - ENSP00000392983 [100%]
ABCB4-201 - ENSP00000351172 [100%]

Other gene match

ANTIGEN VIEW

ABCB4-001	ABCB4-002	ABCB4-003	ABCB4-009	ABCB4-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.