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ITGA2B

ANTIBODY INFORMATION
 
 

Antibody HPA031168

  

Antibody HPA031169

  

Antibody HPA031170

  

Antibody HPA031171

  

Antibody CAB018611

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA031168   HPA031169   HPA031170   HPA031171   sc-15328  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

          

Released in version

 7   7   7   7   4  

References

          

Proper citation

 Atlas Antibodies Cat#HPA031168, RRID:AB_10664706   Atlas Antibodies Cat#HPA031169, RRID:AB_10669671   Atlas Antibodies Cat#HPA031170, RRID:AB_10696214   Atlas Antibodies Cat#HPA031171, RRID:AB_10669773   Santa Cruz Biotechnology Cat#sc-15328, RRID:AB_2265153  

VALIDATION SUMMARY
 
 

Antibody HPA031168

  

Antibody HPA031169

  

Antibody HPA031170

  

Antibody HPA031171

  

Antibody CAB018611

  
Immunocytochemistry

      Approved    
Immunohistochemistry


Validated
   Supported   Supported   Supported   Supported  
Western blot

Uncertain   Uncertain   Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported   Supported   Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA031168

  

Antibody HPA031169

  

Antibody HPA031170

  

Antibody HPA031171

  

Antibody CAB018611

  

Standard validation

       Approved    

         

Figure description

       Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, cytosol & cell junctions.    

Antibody dilution

       1:67    

Literature conformity

       The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA031168

  

Antibody HPA031169

  

Antibody HPA031170

  

Antibody HPA031171

  

Antibody CAB018611

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		          

         

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.          
 

Standard validation

 Supported   Supported   Supported   Supported   Supported  

         

Figure description

 Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in megakaryocytes.   Immunohistochemical staining of human bone marrow shows moderate cytoplasmic positivity in megakaryocytes.   Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in megakaryocytes.   Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in megakaryocytes.   Immunohistochemical staining of human bone marrow shows moderate cytoplasmic positivity in megakaryocytes.  

Expression

 RNA: detected in 14 tissues
Protein: detected in 2 cell types		   RNA: detected in 14 tissues
Protein: detected in 3 cell types		   RNA: detected in 14 tissues
Protein: detected in 6 cell types		   RNA: detected in 14 tissues
Protein: detected in 7 cell types		   RNA: detected in 14 tissues
Protein: detected in 2 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:500   1:500   1:350   1:350   1:175  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA031168

  

Antibody HPA031169

  

Antibody HPA031170

  

Antibody HPA031171

  

Antibody CAB018611

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

         

Figure description

          

Target mass (kDa)

 113.4   113.4   113.4   113.4   113.4, 11.4, 3.5  

Antibody dilution

 1:250   1:250   1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA031168

  

Antibody HPA031169

  

Antibody HPA031170

  

Antibody HPA031171

  

Antibody CAB018611

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

         

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000   1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA031168

  

Antibody HPA031169

  

Antibody HPA031170

  

Antibody HPA031171

  

Antibody CAB018611

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 77   99   93   61    

Antigen sequence

 
CVPQLQLTASVTGSPLLVGADNVLELQMDAANEGEGAYEAELAVHLPQGA
HYMRALSNVEGFERLICNQKKENETRV
  
NDFSWDKRYCEAGFSSVVTQAGELVLGAPGGYYFLGLLAQAPVADIFSSY
RPGILLWHVSSQSLSFDSSNPEYFDGYWGYSVAVGEFDGDLNTTEYVVG
  
GATGHNIPQKLSLNAELQLDRQKPRQGRRVLLLGSQQAGTTLNLDLGGKH
SPICHTTMAFLRDEADFRDKLSPIVLSLNVSLPPTEAGMAPAV
  
FGFSLDFHKDSHGRVAIVVGAPRTLGPSQEETGGVFLCPWRAEGGQCPSL
LFDLRDETRNV
  

											 
																				

																		

										
											
Matching transcripts

												
																					ITGA2B-001 - ENSP00000262407 [100%] 

											 
																						ITGA2B-001 - ENSP00000262407 [100%] 

											 
																						ITGA2B-001 - ENSP00000262407 [100%] 

											 
																						ITGA2B-001 - ENSP00000262407 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ITGA2B-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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