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Immunofluorescent staining of human cell line SH-SY5Y shows localization to nuclear bodies.
Antibody dilution
1:200
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA030608
Antibody HPA061460
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human nasopharynx shows moderate nuclear positivity in respiratory epithelial cells.
Expression
RNA: detected in 6 tissues Protein: detected in 41 cell types
Retrieval
HIER pH6
Antibody dilution
1:200
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA030608
Antibody HPA061460
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
32.6, 18.6
32.6
Antibody dilution
1:250
1:740
PROTEIN ARRAY
Antibody HPA030608
Antibody HPA061460
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3000
1:8850
ANTIGEN INFORMATION
Antibody HPA030608
Antibody HPA061460
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
101
56
Antigen sequence
ALPERAELAASLGLTQTQVKIWFQNKRSKFKKLLKQGSNPHESDPLQGSA
ALSPRSPALPPVWDVSASAKGVSMPPNSYMPGYSHWYSSPHQDTMQRPQM
M
