AASS - Antibodies - The Human Protein Atlas

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AASS

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA020637

Antibody HPA020728

Antibody HPA020734

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA020637

HPA020728

HPA020734

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA020637, RRID:AB_1844415

Atlas Antibodies Cat#HPA020728, RRID:AB_1844416

Atlas Antibodies Cat#HPA020734, RRID:AB_1844417

VALIDATION SUMMARY

Antibody HPA020637

Antibody HPA020728

Antibody HPA020734

Immunocytochemistry

Immunohistochemistry

Supported

Western blot

Supported

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA020637

Antibody HPA020728

Antibody HPA020734

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA020734.
Antibody staining overlaps with antibody HPA020728.

Validated

Antibody staining overlaps with antibody HPA020734.
Antibody staining overlaps with antibody HPA020637.

Validated

Antibody staining overlaps with antibody HPA020728.
Antibody staining overlaps with antibody HPA020637.

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria & vesicles.

Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.

Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria & vesicles.

Antibody dilution

1:43

1:50

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA020637

Antibody HPA020728

Antibody HPA020734

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA020728 across 72 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA020637 across 72 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Expression

RNA: detected in 35 tissues
Protein: detected in 44 cell types

RNA: detected in 35 tissues
Protein: detected in 56 cell types

RNA: detected in 35 tissues
Protein: detected in 23 cell types

Retrieval

HIER pH6

Antibody dilution

1:50

1:200

1:300

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA020637

Antibody HPA020728

Antibody HPA020734

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

102.1

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA020637

Antibody HPA020728

Antibody HPA020734

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

1:3000

1:500

ANTIGEN INFORMATION

Antibody HPA020637

Antibody HPA020728

Antibody HPA020734

Antigen

Recombinant protein fragment

Length (aa)

117

122

132

Antigen sequence

AEWLGLLGDEQVPQAESILDALSKHLVMKLSYGPEEKDMIVMRDSFGIRH
PSGHLEHKTIDLVAYGDINGFSAMAKTVGLPTAMAAKMLLDGEIGAKGLM
GPFSKEIYGPILERIKA

PVSMDICKQEEKLGFLVAKQDLVISLLPYVLHPLVAKACITNKVNMVTAS
YITPALKELEKSVEDAGITIIGELGLDPGLDHMLAMETIDKAKEVGATIE
SYISYCGGLPAPEHSNNPLRYK

MLQVHRTGLGRLGVSLSKGLHHKAVLAVRREDVNAWERRAPLAPKHIKGI
TNLGYKVLIQPSNRRAIHDKDYVKAGGILQEDISEACLILGVKRPPEEKL
MSRKTYAFFSHTIKAQEANMGLLDEILKQEIR

Matching transcripts

AASS-001 - ENSP00000403768 [100%]
AASS-003 - ENSP00000377040 [100%]

Other gene match

ANTIGEN VIEW

AASS-001	AASS-003

RELEVANT PUBLICATIONS

Antibody HPA020637

Antibody HPA020728

Antibody HPA020734

Genetic basis of hyperlysinemia
Houten SM et al
Orphanet J Rare Dis 2013;8:57

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.