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ANTIBODY INFORMATION
 
 

Antibody HPA007533

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA007533  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 12  

References

  

Proper citation

 Atlas Antibodies Cat#HPA007533, RRID:AB_2667855  

VALIDATION SUMMARY
 
 

Antibody HPA007533

  
Immunocytochemistry

Supported  
Immunohistochemistry


Validated
  
Western blot

Supported  
Protein array

Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA007533

  

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.  

Antibody dilution

 1:8  

Literature conformity

 The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA007533

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human skeletal muscle shows nuclear positivity in myocytes.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 59 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:35  

Literature conformity

 Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA007533

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

 

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 44.7  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA007533

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA007533

  

Antigen

 Recombinant protein fragment  

Length (aa)

 145  

Antigen sequence

 
SPSYYNINQVTLGRRSITSPPSTSTTKRPKSIDDSEMESPVDDVFYPGTG
RSPAAGSSQSSGWPNDVDAGSPRATASALHFPSTSIIQQSSPYFTHPTIR
YHHHHGQDSLKEFVQFVCSDGSGQATGQHSQRQAPPLPTGLSASD
 

Matching transcripts

 NFIX-003 - ENSP00000353219 [100%]
NFIX-201 - ENSP00000351354 [100%]
 

Other gene match

  
ANTIGEN VIEW
NFIX-003
NFIX-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.