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Immunofluorescent staining of human cell line SH-SY5Y shows localization to nuclear bodies.
Antibody dilution
1:137
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA067010
Antibody CAB037081
Antibody CAB037158
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
Expression
RNA: detected in 37 tissues Protein: detected in 65 cell types
RNA: detected in 37 tissues Protein: detected in 70 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:900
1:2000
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA067010
Antibody CAB037081
Antibody CAB037158
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
170.9
170.9
170.9
Antibody dilution
1:460
1:500
1:500
PROTEIN ARRAY
Antibody HPA067010
Antibody CAB037081
Antibody CAB037158
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
