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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
Antibody dilution
1:16
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA050007
Antibody CAB033767
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts, Leydig cells exhibited strong cytoplasmic staining.
Immunohistochemical staining of human heart muscle shows distinct cytoplasmic and nuclear positivity in myocytes.
Expression
RNA: detected in 37 tissues Protein: detected in 70 cell types
RNA: detected in 37 tissues Protein: detected in 55 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:125
1:65
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA050007
Antibody CAB033767
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
77.8
77.8
Antibody dilution
1:220
1:500
PROTEIN ARRAY
Antibody HPA050007
Antibody CAB033767
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
