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CD9

ANTIBODY INFORMATION
 
 

Antibody CAB002490

  

Provider

 Novocastra
  

Product name

 NCL-CD9  

Host species

 Mouse  

Clonality

 mAb  

Purity

 Not known  

Other gene match

  

Released in version

 1  

References

  

Proper citation

  

VALIDATION SUMMARY
 
 

Antibody CAB002490

  
Immunocytochemistry

Supported  
Immunohistochemistry


Validated
  
Western blot

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody CAB002490

  

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.  

Antibody dilution

 1:286  

Literature conformity

 The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody CAB002490

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.  
 

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 36 tissues.  
 

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human rectum shows strong cytoplasmic and membranous positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 56 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:300  

Literature conformity

 Consistent with gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody CAB002490

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 25.4, 20.8, 20.6, 17.8, 16  

Antibody dilution

 1:500  

ANTIGEN INFORMATION
 
 

Antibody CAB002490

  

Antigen

 Not known  

Length (aa)

  

Antigen sequence

  

Matching transcripts

  

Other gene match

  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.