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NCAPD2

ANTIBODY INFORMATION
 
 

Antibody HPA036947

  

Antibody HPA037363

  

Antibody CAB012423

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Abcam plc
  

Product name

 HPA036947   HPA037363   34338  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 7   7   4  

References

      

Proper citation

 Atlas Antibodies Cat#HPA036947, RRID:AB_10671367   Atlas Antibodies Cat#HPA037363, RRID:AB_10672483    

VALIDATION SUMMARY
 
 

Antibody HPA036947

  

Antibody HPA037363

  

Antibody CAB012423

  
Immunocytochemistry

  Supported   Supported  
Immunohistochemistry


Validated
  

Validated
   Supported  
Western blot


Validated
   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA036947

  

Antibody HPA037363

  

Antibody CAB012423

  

Standard validation

   Supported   Supported  

     

Figure description

   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & cytosol.  

Antibody dilution

   1:30   1:25  

Literature conformity

   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA036947

  

Antibody HPA037363

  

Antibody CAB012423

  

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA037363 across 45 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA036947 across 45 cell lines.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human tonsil shows strong cytoplasmic and nuclear positivity in reaction center cells.   Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferus ducts and Leydig cells.   Immunohistochemical staining of human liver shows distinct nuclear positivity in hepatocytes.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 50 cell types		   RNA: detected in 37 tissues
Protein: detected in 65 cell types		   RNA: detected in 37 tissues
Protein: detected in 77 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:10   1:35   1:100  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA036947

  

Antibody HPA037363

  

Antibody CAB012423

  

Formal validation: Genetic
Validated

Downregulation visible in both siRNA lanes		      

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		      

Target mass (kDa)

 157.2, 102.6      

Loading control

 Total protein image      

Antibody dilution

 1:58      
 

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 157.2, 102.6   157.2, 102.6   157.2, 102.6, 13.3  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA036947

  

Antibody HPA037363

  

Antibody CAB012423

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA036947

  

Antibody HPA037363

  

Antibody CAB012423

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 94   106    

Antigen sequence

 
DKSVLVCKNAIQLLASFLANNPFSCKLSDADLAGPLQKETQKLQEMRAQR
RTAAASAVLDPEEEWEAMLPELKSTLQQLLQLPQGEEEIPEQIA
  
VGTIQCLEEILCEFVQKDELKPAVTQLLWERATEKVACCPLERCSSVMLL
GMMARGKPEIVGSNLDTLVSIGLDEKFPQDYRLAQQVCHAIANISDRRKP
SLGKRH
  

											 
																				

																		

										
											
Matching transcripts

												
																					NCAPD2-001 - ENSP00000325017 [100%] 
NCAPD2-002 - ENSP00000371895 [100%] 

											 
																						NCAPD2-001 - ENSP00000325017 [100%] 
NCAPD2-002 - ENSP00000371895 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
NCAPD2-001
NCAPD2-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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