Fields »
ABOUT
HELP
BLOG
ANLN

ANTIBODY INFORMATION
 
 

Antibody HPA005680

  

Antibody HPA050556

  

Antibody CAB033902

  

Antibody CAB062547

  

Antibody CAB068175

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA005680   HPA050556   sc-67327   AMAb90660   AMAb90662  

Host species

 Rabbit   Rabbit   Rabbit   Mouse   Mouse  

Clonality

 pAb   pAb   pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Protein A/G   Protein A/G  

Other gene match

          

Released in version

 12   12   13   12   13  

References

 2          

Proper citation

 Atlas Antibodies Cat#HPA005680, RRID:AB_2667388   Atlas Antibodies Cat#HPA050556, RRID:AB_2681175   Santa Cruz Biotechnology Cat#sc-67327, RRID:AB_2058302   Atlas Antibodies Cat#AMAb90660, RRID:AB_2665622   Atlas Antibodies Cat#AMAb90662, RRID:AB_2665623  

VALIDATION SUMMARY
 
 

Antibody HPA005680

  

Antibody HPA050556

  

Antibody CAB033902

  

Antibody CAB062547

  

Antibody CAB068175

  
Immunocytochemistry

  Supported        
Immunohistochemistry


Validated
  

Validated
   Supported   Supported   Supported  
Western blot

Supported   Supported   Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported        

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA005680

  

Antibody HPA050556

  

Antibody CAB033902

  

Antibody CAB062547

  

Antibody CAB068175

  

Standard validation

   Supported        

         

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.        

Antibody dilution

   1:60        

Literature conformity

   The subcellular location is supported by literature.        

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA005680

  

Antibody HPA050556

  

Antibody CAB033902

  

Antibody CAB062547

  

Antibody CAB068175

  

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		        

 
       

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA050556 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA005680 across 46 cell lines.		        
 

Standard validation

 Supported   Supported   Supported   Supported   Supported  

         

Figure description

 Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.   Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.   Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.   Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.   Immunohistochemical staining of human duodenum shows nuclear positivity in glandular cells.  

Expression

 RNA: detected in 27 tissues
Protein: detected in 55 cell types		   RNA: detected in 27 tissues
Protein: detected in 32 cell types		   RNA: detected in 27 tissues
Protein: detected in 24 cell types		   RNA: detected in 27 tissues
Protein: detected in 46 cell types		   RNA: detected in 27 tissues
Protein: detected in 36 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:100   1:450   1:125   1:100   1:100  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA005680

  

Antibody HPA050556

  

Antibody CAB033902

  

Antibody CAB062547

  

Antibody CAB068175

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

         

Figure description

 Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		        

Target mass (kDa)

 124.2, 120   124.2, 120   124.2, 120, 30.8, 27.8, 26.6, 18.2, 3.8   124.2, 120, 30.8, 27.8, 26.6, 18.2, 3.8   124.2, 120, 30.8, 27.8, 26.6, 18.2, 3.8  

Antibody dilution

 1:250   1:300   1:500   1:500   1:1000  

PROTEIN ARRAY
 
 

Antibody HPA005680

  

Antibody HPA050556

  

Antibody CAB033902

  

Antibody CAB062547

  

Antibody CAB068175

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		        

         

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.        

Antibody dilution

 1:1000   1:2400        

ANTIGEN INFORMATION
 
 

Antibody HPA005680

  

Antibody HPA050556

  

Antibody CAB033902

  

Antibody CAB062547

  

Antibody CAB068175

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein   Recombinant protein   Recombinant protein  

Length (aa)

 136   133        

Antigen sequence

 
IVKSTLSQTVPSKGELSREICLQSQSKDKSTTPGGTGIKPFLERFGERCQ
EHSKESPARSTPHRTPIITPNTKAIQERLFKQDTSSSTTHLAQQLKQERQ
KELACLRGRFDKGNIWSAEKGGNSKSKQLETKQETH
  
DLFSDVLEEGELDMEKSQEEMDQALAESSEEQEDALNISSMSLLAPLAQT
VGVVSPESLVSTPRLELKDTSRSDESPKPGKFQRTRVPRAESGDSLGSED
RDLLYSIDAYRSQRFKETERPSIKQVIVRKEDV
  

											 
																						

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					ANLN-001 - ENSP00000265748 [100%] 
ANLN-002 - ENSP00000379380 [100%] 

											 
																						ANLN-001 - ENSP00000265748 [100%] 
ANLN-002 - ENSP00000379380 [100%] 

											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ANLN-001
ANLN-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA005680

												
										 
																				
											
											
											
Antibody HPA050556

												
										 
																				
											
											
											
Antibody CAB033902

												
										 
																				
											
											
											
Antibody CAB062547

												
										 
																				
											
											
											
Antibody CAB068175

												
										 
																		

																	

										
											
1.

												
																						Systematic antibody generation and validation via tissue microarray technology leading to identification of a novel protein prognostic panel in breast cancer

	O Leary PC et al

	BMC Cancer 2013;13:175

			
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						Generation of monospecific antibodies based on affinity capture of polyclonal antibodies

	Hjelm B et al

	Protein Sci 2011;20(11):1824-35

			
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

					Contact
				

				
				

					
					
					
					
				

				
			


			

				

					The Project
				
			
				
			

			
			

			
				

					The Human Protein Atlas
				

				
			

			
			

			

						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.