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Immunohistochemical staining of human hippocampus shows positivity in neuropil.
Expression
RNA: detected in 4 tissues Protein: detected in 10 cell types
Retrieval
HIER pH6
Antibody dilution
1:850
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Standard validation
Supported
Figure description
Strong labelling of various synaptic fields throughout the brain including ventral pallidum.
WESTERN BLOT
Antibody HPA000839
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY424511)
Target mass (kDa)
55.2
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA000839
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
