Fields »
ABOUT
HELP
BLOG
MDH1

GENERAL INFORMATION

Gene name

 MDH1

Gene description

 Malate dehydrogenase 1, NAD (soluble)

Protein class

 Cancer-related genes
Citric acid cycle related proteins
Enzymes
Plasma proteins
Predicted intracellular proteins

Predicted localization

 Intracellular

Number of transcripts

 9
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category
TCGA (cancer tissue):Expressed in all
HPA (cell line):Expressed in all
HPA (normal tissue):Expressed in all

Protein evidence

 Evidence at protein level

Protein expression
normal tissue

 Cytoplasmic expression in several different tissue types.

ANTIBODY IHC RELIABILITY

Data reliability
description

 Antibody staining mainly not consistent with RNA expression data.

Reliability score

 Uncertain based on 3 antibodies.
HPA027296 , HPA054276 , CAB047333
SHOW MORE

PROGNOSTIC SUMMARY
Prognostic marker in endometrial cancer (unfavourable) and renal cancer (favourable).
Endometrial cancer p<0.001
Renal cancer p<0.001
Show all

RNA EXPRESSION OVERVIEW
TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION
Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY
HPA027296
HPA054276
CAB047333

GENE INFORMATION

Gene name

 MDH1

Synonyms

Description

 Malate dehydrogenase 1, NAD (soluble) (HGNC Symbol)

Entrez gene summary

 This gene encodes an enzyme that catalyzes the reversible oxidation of malate to oxaloacetate using the NAD/NADH cofactor system in the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one found in the mitochondrial matrix and the other in the cytoplasm. This protein represents the cytosolic isozyme, which has a pivotal role in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. A recent study showed that two protein isoforms are produced from this mRNA by use of alternative in-frame translation termination codons via a stop codon readthrough mechanism, and that these C-terminally distinct isoforms have different subcellular localization. Alternatively spliced transcript variants have also been found for this gene. Pseudogenes have been identified on chromosomes X and 6. [provided by RefSeq, Oct 2015]

Chromosome

 2

Cytoband

 p15

Chromosome location (bp)

 63588609 - 63607197

Protein evidence

 Evidence at protein level (all genes)

Ensembl

 ENSG00000014641 (version 83.38)

Entrez gene

 4190

UniProt

 P40925 (UniProt - Evidence at protein level)

neXtProt

 NX_P40925

Antibodypedia

 MDH1 antibodies

PROTEIN BROWSER
MDH1-001
MDH1-003
MDH1-004
MDH1-006
MDH1-007
MDH1-010
MDH1-011
MDH1-201
MDH1-202

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)
MDH1-001
ENSP00000233114
ENST00000233114
P40925 [Direct mapping]
Malate dehydrogenase, cytoplasmic
V9HWF2 [Target identity:100%; Query identity:100%]
Malate dehydrogenase
Show all
Enzymes
   ENZYME proteins
   Oxidoreductases
   SPOCTOPUS predicted membrane proteins
Predicted intracellular proteins
Plasma proteins
Citric acid cycle related proteins
Cancer-related genes
   Candidate cancer biomarkers
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0002230 [positive regulation of defense response to virus by host]
GO:0003824 [catalytic activity]
GO:0004470 [malic enzyme activity]
GO:0005615 [extracellular space]
GO:0005737 [cytoplasm]
GO:0005813 [centrosome]
GO:0005829 [cytosol]
GO:0005975 [carbohydrate metabolic process]
GO:0006006 [glucose metabolic process]
GO:0006094 [gluconeogenesis]
GO:0006099 [tricarboxylic acid cycle]
GO:0006108 [malate metabolic process]
GO:0016491 [oxidoreductase activity]
GO:0016615 [malate dehydrogenase activity]
GO:0016616 [oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor]
GO:0019752 [carboxylic acid metabolic process]
GO:0030060 [L-malate dehydrogenase activity]
GO:0044281 [small molecule metabolic process]
GO:0047860 [diiodophenylpyruvate reductase activity]
GO:0055114 [oxidation-reduction process]
GO:0070062 [extracellular exosome]
GO:0098779 [mitophagy in response to mitochondrial depolarization]
Show all
 334 aa
36.4 kDa 		No 0
MDH1-003
ENSP00000410073
ENST00000432309
C9JF79 [Direct mapping]
Malate dehydrogenase
Show all
   SPOCTOPUS predicted membrane proteins
Predicted intracellular proteins
Citric acid cycle related proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003824 [catalytic activity]
GO:0005737 [cytoplasm]
GO:0005813 [centrosome]
GO:0005975 [carbohydrate metabolic process]
GO:0006099 [tricarboxylic acid cycle]
GO:0006108 [malate metabolic process]
GO:0016491 [oxidoreductase activity]
GO:0016615 [malate dehydrogenase activity]
GO:0016616 [oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor]
GO:0030060 [L-malate dehydrogenase activity]
GO:0055114 [oxidation-reduction process]
Show all
 222 aa
24.2 kDa 		No 0
MDH1-004
ENSP00000394504
ENST00000436321
C9JRL4 [Direct mapping]
Malate dehydrogenase, cytoplasmic
Show all
Predicted intracellular proteins
Citric acid cycle related proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0006108 [malate metabolic process]
GO:0016491 [oxidoreductase activity]
GO:0016615 [malate dehydrogenase activity]
GO:0055114 [oxidation-reduction process]
Show all
 120 aa
13 kDa 		No 0
MDH1-006
ENSP00000399004
ENST00000442225
C9IZI0 [Direct mapping]
Malate dehydrogenase, cytoplasmic
Show all
   Phobius predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
Predicted intracellular proteins
Citric acid cycle related proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0006108 [malate metabolic process]
GO:0016491 [oxidoreductase activity]
GO:0016615 [malate dehydrogenase activity]
GO:0055114 [oxidation-reduction process]
Show all
 67 aa
7.1 kDa 		No 0
MDH1-007
ENSP00000409027
ENST00000454035
C9JLV6 [Direct mapping]
Malate dehydrogenase, cytoplasmic
Show all
   SPOCTOPUS predicted membrane proteins
Predicted intracellular proteins
Citric acid cycle related proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005737 [cytoplasm]
GO:0005813 [centrosome]
GO:0006108 [malate metabolic process]
GO:0016491 [oxidoreductase activity]
GO:0016615 [malate dehydrogenase activity]
GO:0055114 [oxidation-reduction process]
Show all
 114 aa
12.4 kDa 		No 0
MDH1-010
ENSP00000386743
ENST00000409908
B8ZZ51 [Direct mapping]
Malate dehydrogenase, cytoplasmic
Show all
Predicted intracellular proteins
Citric acid cycle related proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003824 [catalytic activity]
GO:0005975 [carbohydrate metabolic process]
GO:0006108 [malate metabolic process]
GO:0016615 [malate dehydrogenase activity]
GO:0016616 [oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor]
GO:0055114 [oxidation-reduction process]
Show all
 169 aa
18.7 kDa 		No 0
MDH1-011
ENSP00000386719
ENST00000409476
B9A041 [Direct mapping]
Malate dehydrogenase, cytoplasmic
Show all
Predicted intracellular proteins
Citric acid cycle related proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003824 [catalytic activity]
GO:0005975 [carbohydrate metabolic process]
GO:0006108 [malate metabolic process]
GO:0016615 [malate dehydrogenase activity]
GO:0016616 [oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor]
GO:0055114 [oxidation-reduction process]
Show all
 210 aa
23 kDa 		No 0
MDH1-201
ENSP00000438144
ENST00000539945
P40925 [Direct mapping]
Malate dehydrogenase, cytoplasmic
Show all
Enzymes
   ENZYME proteins
   Oxidoreductases
   SPOCTOPUS predicted membrane proteins
Predicted intracellular proteins
Plasma proteins
Citric acid cycle related proteins
Cancer-related genes
   Candidate cancer biomarkers
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0002230 [positive regulation of defense response to virus by host]
GO:0003824 [catalytic activity]
GO:0004470 [malic enzyme activity]
GO:0005615 [extracellular space]
GO:0005737 [cytoplasm]
GO:0005739 [mitochondrion]
GO:0005813 [centrosome]
GO:0005829 [cytosol]
GO:0005975 [carbohydrate metabolic process]
GO:0006006 [glucose metabolic process]
GO:0006094 [gluconeogenesis]
GO:0006099 [tricarboxylic acid cycle]
GO:0006107 [oxaloacetate metabolic process]
GO:0006108 [malate metabolic process]
GO:0006734 [NADH metabolic process]
GO:0016491 [oxidoreductase activity]
GO:0016615 [malate dehydrogenase activity]
GO:0016616 [oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor]
GO:0019674 [NAD metabolic process]
GO:0019752 [carboxylic acid metabolic process]
GO:0030060 [L-malate dehydrogenase activity]
GO:0043209 [myelin sheath]
GO:0044281 [small molecule metabolic process]
GO:0047860 [diiodophenylpyruvate reductase activity]
GO:0051287 [NAD binding]
GO:0055114 [oxidation-reduction process]
GO:0070062 [extracellular exosome]
GO:0098779 [mitophagy in response to mitochondrial depolarization]
Show all
 352 aa
38.6 kDa 		No 0
MDH1-202
ENSP00000446395
ENST00000544381
P40925 [Direct mapping]
Malate dehydrogenase, cytoplasmic
Show all
Enzymes
   ENZYME proteins
   Oxidoreductases
Predicted intracellular proteins
Plasma proteins
Citric acid cycle related proteins
Cancer-related genes
   Candidate cancer biomarkers
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0002230 [positive regulation of defense response to virus by host]
GO:0003824 [catalytic activity]
GO:0004470 [malic enzyme activity]
GO:0005615 [extracellular space]
GO:0005737 [cytoplasm]
GO:0005813 [centrosome]
GO:0005829 [cytosol]
GO:0005975 [carbohydrate metabolic process]
GO:0006006 [glucose metabolic process]
GO:0006094 [gluconeogenesis]
GO:0006099 [tricarboxylic acid cycle]
GO:0006108 [malate metabolic process]
GO:0016491 [oxidoreductase activity]
GO:0016615 [malate dehydrogenase activity]
GO:0016616 [oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor]
GO:0030060 [L-malate dehydrogenase activity]
GO:0044281 [small molecule metabolic process]
GO:0047860 [diiodophenylpyruvate reductase activity]
GO:0055114 [oxidation-reduction process]
GO:0070062 [extracellular exosome]
GO:0098779 [mitophagy in response to mitochondrial depolarization]
Show all
 245 aa
27 kDa 		No 0

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.