BID - Antibodies - The Human Protein Atlas

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BID

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA000722

Antibody CAB003771

Provider

Atlas Antibodies
Sigma-Aldrich

Epitomics

Product name

HPA000722

1008-1

Host species

Rabbit

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Supernatant

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA000722, RRID:AB_1078283

VALIDATION SUMMARY

Antibody HPA000722

Antibody CAB003771

Immunocytochemistry

Supported

Immunohistochemistry

Western blot

Supported

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA000722

Antibody CAB003771

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to cytosol.

Antibody dilution

1:154

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA000722

Antibody CAB003771

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.

Immunohistochemical staining of human lymph node shows moderate cytoplasmic positivity.

Expression

RNA: detected in 37 tissues
Protein: detected in 34 cell types

RNA: detected in 37 tissues
Protein: detected in 33 cell types

Retrieval

HIER pH6

Antibody dilution

1:1500

1:25

Literature conformity

Partly consistent with extensive gene/protein characterization data.

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA000722

Antibody CAB003771

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT4
Lane 3: EFO-21
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

26.8, 22

26.8, 22, 14.6, 13, 11.3

Antibody dilution

1:500

PROTEIN ARRAY

Antibody HPA000722

Antibody CAB003771

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

ANTIGEN INFORMATION

Antibody HPA000722

Antibody CAB003771

Antigen

Recombinant protein fragment

Synthetic peptide

Length (aa)

187

Antigen sequence

VNNGSSLRDECITNLLVFGFLQSCSDNSFRRELDALGHELPVLAPQWEGY
DELQTDGNRSSHSRLGRIEADSESQEDIIRNIARHLAQVGDSMDRSIPPG
LVNGLALQLRNTSRSEEDRNRDLATALEQLLQAYPRDMEKEKTMLVLALL
LAKKVASHTPSLLRDVFHTTVNFINQNLRTYVRSLAR

Matching transcripts

BID-001 - ENSP00000382674 [100%]
BID-002 - ENSP00000318822 [100%]
BID-009 - ENSP00000449236 [100%]
BID-206 - ENSP00000480414 [100%]

Other gene match

ANTIGEN VIEW