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Immunofluorescent staining of human cell line U-2 OS shows localization to peroxisomes.
Immunofluorescent staining of human cell line A-431 shows localization to nucleoli & peroxisomes.
Antibody dilution
1:81
1:103
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA030209
Antibody HPA030210
Antibody HPA030211
Formal validation: Independent
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA030210 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA030211 across 46 cell lines. Pearson correlation with HPA030209 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA030210 across 46 cell lines.
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA030211 across 74 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA030210 across 74 cell types.
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human lateral ventricle shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human epididymis shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in subsets of islet cells.
Expression
RNA: detected in 37 tissues Protein: detected in 41 cell types
RNA: detected in 37 tissues Protein: detected in 30 cell types
RNA: detected in 37 tissues Protein: detected in 48 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:250
1:100
1:325
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Consistent with gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA030209
Antibody HPA030210
Antibody HPA030211
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
72.9
72.9
72.9
Antibody dilution
1:250
1:250
1:250
PROTEIN ARRAY
Antibody HPA030209
Antibody HPA030210
Antibody HPA030211
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
