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ADGRA2

ANTIBODY INFORMATION
 
 

Antibody HPA012393

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA012393  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 4  

References

  

Proper citation

 Atlas Antibodies Cat#HPA012393, RRID:AB_1849256  

VALIDATION SUMMARY
 
 

Antibody HPA012393

  
Immunohistochemistry

Uncertain  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA012393

  

Standard validation

 Uncertain  

 

Figure description

 Immunohistochemical staining of human liver shows strong membranous positivity in hepatocytes.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 13 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:25  

Literature conformity

 Not consistent with gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA012393

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

 

Figure description

  

Target mass (kDa)

 142.6, 119.9  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA012393

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA012393

  

Antigen

 Recombinant protein fragment  

Length (aa)

 109  

Antigen sequence

 
SPTDSYLGSSRNSPGAGLQLEGEPMLTPSEGSDTSAAPLSEAGRAGQRRS
ASRDSLKGGGALEKESHRRSYPLNAASLNGAPKGGKYDDVTLMGAEVASG
GCMKTGLWK
 

Matching transcripts

 ADGRA2-001 - ENSP00000406367 [100%]
ADGRA2-002 - ENSP00000323508 [100%]
 

Other gene match

  
ANTIGEN VIEW
ADGRA2-001
ADGRA2-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.