SERPINB1 - Antibodies - The Human Protein Atlas

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SERPINB1

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA018871

Antibody HPA052642

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA018871

HPA052642

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA018871, RRID:AB_1856724

Atlas Antibodies Cat#HPA052642, RRID:AB_2681896

VALIDATION SUMMARY

Antibody HPA018871

Antibody HPA052642

Immunohistochemistry

Supported

Western blot

Supported

Protein array

Approved

IMMUNOHISTOCHEMISTRY

Antibody HPA018871

Antibody HPA052642

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 36 tissues.

Standard validation

Supported

Figure description

Immunohistochemical staining of human spleen shows moderate cytoplasmic positivity in cells in red pulp.

Immunohistochemical staining of human bone marrow shows moderate cytoplasmic positivity in hematopoietic cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 16 cell types

RNA: detected in 37 tissues
Protein: detected in 5 cell types

Retrieval

HIER pH6

Antibody dilution

1:12000

1:1000

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA018871

Antibody HPA052642

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

42.7

Antibody dilution

1:250

1:80

PROTEIN ARRAY

Antibody HPA018871

Antibody HPA052642

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:900

ANTIGEN INFORMATION

Antibody HPA018871

Antibody HPA052642

Antigen

Recombinant protein fragment

Length (aa)

109

Antigen sequence

GNWKDKFMKEATTNAPFRLNKKDRKTVKMMYQKKKFAYGYIEDLKCRVLE
LPYQGEELSMVILLPDDIEDESTGLKKIEEQLTLEKLHEWTKPENLDFIE
VNVSLPRFK

YNFLPEFLVSTQKTYGADLASVDFQHASEDARKTINQWVKGQTEGKIPEL
LASGMVDNMTKLVLVN

Matching transcripts

SERPINB1-003 - ENSP00000370115 [100%]

Other gene match

ANTIGEN VIEW

SERPINB1-003

RELEVANT PUBLICATIONS

Antibody HPA018871

Antibody HPA052642

Proteomic analysis reveals heat shock protein 70 has a key role in polycythemia Vera
Gallardo M et al
Mol Cancer 2013;12:142
Application: IHC

SERPINB1 expression is predictive for sensitivity and outcome of cisplatin-based chemotherapy in melanoma
Willmes C et al
Oncotarget 2016;7(9):10117-32
Application: IHC

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.