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ANTIBODY INFORMATION
 
 

Antibody HPA040624

  

Antibody HPA055647

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA040624   HPA055647  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 16   12  

References

    

Proper citation

 Atlas Antibodies Cat#HPA040624, RRID:AB_2677050   Atlas Antibodies Cat#HPA055647, RRID:AB_2682874  

VALIDATION SUMMARY
 
 

Antibody HPA040624

  

Antibody HPA055647

  
Immunocytochemistry


Validated
  

Validated
  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA040624

  

Antibody HPA055647

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA055647.		  
Validated

Antibody staining overlaps with antibody HPA040624.		  
 

Standard validation

 Supported   Approved  

   

Figure description

 Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.  

Antibody dilution

 1:24   1:114  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is partly supported by literature or no literature is available.  

WESTERN BLOT
 
 

Antibody HPA040624

  

Antibody HPA055647

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

    

Target mass (kDa)

 171.3   171.3  

Antibody dilution

 1:250   1:380  

PROTEIN ARRAY
 
 

Antibody HPA040624

  

Antibody HPA055647

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:4550  

ANTIGEN INFORMATION
 
 

Antibody HPA040624

  

Antibody HPA055647

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 121   105  

Antigen sequence

 
SEPVTMDKVHYCERFIELMIDLEALLPTRRWFNTILDDSHLLVHCYLSNL
VRREEDGHLFSQLLDMLKFYTGFEINDQTGNALTENEMTTIHYDRITSLQ
RAAFAHFPELYDFALSNVAEV
  
DEWEGLRKHDVCFLITVRPTKPYGTKFDRRRPFIEQVGLVYVRGCEIQGM
LDDKGRVIEDGPEPRPNLRGESRTFRVFLDPNQYQQDMTNTIQNGAEDVY
ETFNI
 

Matching transcripts

 AQR-001 - ENSP00000156471 [100%]
  AQR-001 - ENSP00000156471 [100%]
 

Other gene match

    
ANTIGEN VIEW
AQR-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.