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Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
Antibody dilution
1:69
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA002317
Standard validation
Supported
Figure description
Immunohistochemical staining of human tonsil shows nuclear positivity in reaction center cells.
Expression
RNA: detected in 36 tissues Protein: detected in 32 cell types
Retrieval
HIER pH6
Antibody dilution
1:150
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Standard validation
Approved
Figure description
Cytoplasmic (and in some cases nuclear too) staining in subsets of neurons, most abundant (but not exclusive) in areas of the forebrain and midbrain among others.
WESTERN BLOT
Antibody HPA002317
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY420093)
Target mass (kDa)
68.4
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA002317
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
