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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

GCLM

Gene description

Glutamate-cysteine ligase, modifier subunit

Protein class

Cancer-related genes
Predicted intracellular proteins

Predicted localization

Intracellular

Number of transcripts

2
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Expressed in all
HPA (normal tissue):Expressed in all

Protein evidence

Evidence at protein level

Protein expression
normal tissue

General cytoplasmic expression.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly not consistent with RNA expression data. Pending external verification.

Reliability score

Uncertain based on 3 antibodies.
HPA023696 , CAB009568 , CAB040554
SHOW MORE

PROGNOSTIC SUMMARY

Prognostic marker in liver cancer (unfavourable).
Liver cancer p<0.001
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY

HPA023696
CAB009568
CAB040554

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Most cancers displayed moderate positivity. Strong staining was observed in prostate cancers and cases of urothelial, breast, thyroid, endometrial and squamous cell carcinomas. A majority of melanomas, skin and pancreatic cancers were negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Most cancer tissues displayed weak to moderate cytoplasmic staining. A majority of lymphomas and renal cancers were negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Moderate cytoplasmic immunoreactivity was observed in several cases of lymphomas, melanomas, colorectal, cervical, endometrial and urothelial cancers. Additional nuclear staining was observed in squamous cell carcinomas, colorectal and urothelial cancers. A few cases of liver cancers displayed strong staining. Other cancer tissues were in general weakly stained or negative.

GENE INFORMATION

Gene name

GCLM (HGNC Symbol)

Synonyms

GLCLR

Description

Glutamate-cysteine ligase, modifier subunit (HGNC Symbol)

Entrez gene summary

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]

Chromosome

1

Cytoband

p22.1

Chromosome location (bp)

93885205 - 93909456

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000023909 (version 83.38)

Entrez gene

2730

UniProt

P48507 (UniProt - Evidence at protein level)

neXtProt

NX_P48507

Antibodypedia

GCLM antibodies

PROTEIN BROWSER

GCLM-001
GCLM-201

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

GCLM-001
ENSP00000359258
ENST00000370238
P48507 [Direct mapping]
Glutamate--cysteine ligase regulatory subunit
Show all
Predicted intracellular proteins
Cancer-related genes
   Candidate cancer biomarkers
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000096 [sulfur amino acid metabolic process]
GO:0004357 [glutamate-cysteine ligase activity]
GO:0005829 [cytosol]
GO:0006534 [cysteine metabolic process]
GO:0006536 [glutamate metabolic process]
GO:0006749 [glutathione metabolic process]
GO:0006750 [glutathione biosynthetic process]
GO:0006805 [xenobiotic metabolic process]
GO:0006979 [response to oxidative stress]
GO:0008637 [apoptotic mitochondrial changes]
GO:0017109 [glutamate-cysteine ligase complex]
GO:0030234 [enzyme regulator activity]
GO:0034641 [cellular nitrogen compound metabolic process]
GO:0035226 [glutamate-cysteine ligase catalytic subunit binding]
GO:0035229 [positive regulation of glutamate-cysteine ligase activity]
GO:0042493 [response to drug]
GO:0043524 [negative regulation of neuron apoptotic process]
GO:0044281 [small molecule metabolic process]
GO:0046982 [protein heterodimerization activity]
GO:0050880 [regulation of blood vessel size]
GO:0051409 [response to nitrosative stress]
GO:0051900 [regulation of mitochondrial depolarization]
GO:1901687 [glutathione derivative biosynthetic process]
GO:2001237 [negative regulation of extrinsic apoptotic signaling pathway]
Show all
274 aa
30.7 kDa
No 0
GCLM-201
ENSP00000484507
ENST00000615724
P48507 [Direct mapping]
Glutamate--cysteine ligase regulatory subunit
Show all
Predicted intracellular proteins
Cancer-related genes
   Candidate cancer biomarkers
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000096 [sulfur amino acid metabolic process]
GO:0004357 [glutamate-cysteine ligase activity]
GO:0005829 [cytosol]
GO:0006536 [glutamate metabolic process]
GO:0006750 [glutathione biosynthetic process]
GO:0006805 [xenobiotic metabolic process]
GO:0006979 [response to oxidative stress]
GO:0017109 [glutamate-cysteine ligase complex]
GO:0030234 [enzyme regulator activity]
GO:0034641 [cellular nitrogen compound metabolic process]
GO:0035226 [glutamate-cysteine ligase catalytic subunit binding]
GO:0035229 [positive regulation of glutamate-cysteine ligase activity]
GO:0042493 [response to drug]
GO:0044281 [small molecule metabolic process]
GO:0050880 [regulation of blood vessel size]
GO:1901687 [glutathione derivative biosynthetic process]
Show all
252 aa
28.1 kDa
No 0

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