Fields »
ABOUT
HELP
BLOG
VIM

ANTIBODY INFORMATION
 
 

Antibody HPA001762

  

Antibody CAB000080

  

Antibody CAB058687

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   DakoCytomation
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA001762   M7020   AMAb90516  

Host species

 Rabbit   Mouse   Mouse  

Clonality

 pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G   Protein A/G  

Other gene match

      

Released in version

 2   1   11  

References

 5      

Proper citation

 Atlas Antibodies Cat#HPA001762, RRID:AB_1080568   Dako Cat#M7020, RRID:AB_2304493   Atlas Antibodies Cat#AMAb90516, RRID:AB_2665570  

VALIDATION SUMMARY
 
 

Antibody HPA001762

  

Antibody CAB000080

  

Antibody CAB058687

  
Immunocytochemistry

Approved      
Immunohistochemistry


Validated
  

Validated
  

Validated
  
Western blot

Supported   Supported   Supported  
Protein array

Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001762

  

Antibody CAB000080

  

Antibody CAB058687

  

Standard validation

 Approved      

     

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to intermediate filaments.      

Antibody dilution

 1:74      

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001762

  

Antibody CAB000080

  

Antibody CAB058687

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human rectum shows strong positivity in tissue stroma.   Immunohistochemical staining of human rectum shows strong positivity in tissue stroma.   Immunohistochemical staining of human rectum shows strong positivity in tissue stroma.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 47 cell types		   RNA: detected in 37 tissues
Protein: detected in 48 cell types		   RNA: detected in 37 tissues
Protein: detected in 47 cell types		  

Retrieval

 HIER pH6   HIER pH9   HIER pH6  

Antibody dilution

 1:1000   1:400   1:5000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001762

  

Antibody CAB000080

  

Antibody CAB058687

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 53.7   53.7, 20   53.7, 20  

Antibody dilution

 1:250   1:750   1:500  

PROTEIN ARRAY
 
 

Antibody HPA001762

  

Antibody CAB000080

  

Antibody CAB058687

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000      

ANTIGEN INFORMATION
 
 

Antibody HPA001762

  

Antibody CAB000080

  

Antibody CAB058687

  

Antigen

 Recombinant protein fragment   Native protein   Recombinant protein  

Length (aa)

 113      

Antigen sequence

 
FANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQ
LTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAENTLQSFRQDVDNA
SLARLDLERKVES
     

Matching transcripts

 VIM-001 - ENSP00000224237 [100%]
VIM-201 - ENSP00000446007 [100%]
     

Other gene match

      
ANTIGEN VIEW
VIM-001
VIM-201

RELEVANT PUBLICATIONS
 
 

Antibody HPA001762

  

Antibody CAB000080

  

Antibody CAB058687

  

1.

 Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy
Stadler C et al
J Proteomics 2012;75(7):2236-51
      

2.

 Tumor suppressive microRNA‑138 contributes to cell migration and invasion through its targeting of vimentin in renal cell carcinoma
Yamasaki T et al
Int J Oncol 2012;41(3):805-17
      

3.

 Changes in cannabinoid receptors, aquaporin 4 and vimentin expression after traumatic brain injury in adolescent male mice. Association with edema and neurological deficit
Lopez-Rodriguez AB et al
PLoS One 2015;10(6):e0128782
Application: WB 		     

4.

 Inhibition of Lysyl Oxidase and Lysyl Oxidase-Like Enzymes Has Tumour-Promoting and Tumour-Suppressing Roles in Experimental Prostate Cancer
Nilsson M et al
Sci Rep 2016;6:19608
Application: IHC 		     

5.

 Regulation of glutamine carrier proteins by RNF5 determines breast cancer response to ER stress-inducing chemotherapies
Jeon YJ et al
Cancer Cell 2015;27(3):354-69
Application: IHC 		     
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.