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CD44

ANTIBODY INFORMATION
 
 

Antibody HPA005785

  

Antibody CAB000112

  

Antibody CAB000316

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Chemicon
   DakoCytomation
  

Product name

 HPA005785   MAB4073   M7082  

Host species

 Rabbit   Mouse   Mouse  

Clonality

 pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known   Supernatant  

Other gene match

      

Released in version

 3   2   1  

References

 9      

Proper citation

 Atlas Antibodies Cat#HPA005785, RRID:AB_1078467     Dako Cat#M7082, RRID:AB_2076596  

VALIDATION SUMMARY
 
 

Antibody HPA005785

  

Antibody CAB000112

  

Antibody CAB000316

  
Immunocytochemistry

Supported   Supported   Supported  
Immunohistochemistry


Validated
   Supported   Supported  
Western blot

Supported   Supported   Supported  
Protein array

Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA005785

  

Antibody CAB000112

  

Antibody CAB000316

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.   Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.   Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & the Golgi apparatus.  

Antibody dilution

 1:31   1:400   1:100  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA005785

  

Antibody CAB000112

  

Antibody CAB000316

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		      

     

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.      
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human vagina shows strong cytoplasmic positivity in squamous epithelial cells.   Immunohistochemical staining of human vagina shows strong cytoplasmic positivity in squamous epithelial cells.   Immunohistochemical staining of human vagina shows strong cytoplasmic positivity in squamous epithelial cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 51 cell types		   RNA: detected in 37 tissues
Protein: detected in 15 cell types		   RNA: detected in 37 tissues
Protein: detected in 56 cell types		  

Retrieval

 HIER pH6   HIER pH9   HIER pH9  

Antibody dilution

 1:300   1:500   1:100  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA005785

  

Antibody CAB000112

  

Antibody CAB000316

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 81.5, 26.7   81.5, 76.6, 53.4, 46.6, 39.4, 37.3, 31.9, 31, 27.3, 26.7, 26, 25.7, 24.7, 22.7, 21.5, 21.2, 21.1, 21, 20.1, 19.5, 15.6, 13.5, 9.1, 8.8   81.5, 76.6, 53.4, 46.6, 39.4, 37.3, 31.9, 31, 27.3, 26.7, 26, 25.7, 24.7, 22.7, 21.5, 21.2, 21.1, 21, 20.1, 19.5, 15.6, 13.5, 9.1, 8.8  

Antibody dilution

 1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA005785

  

Antibody CAB000112

  

Antibody CAB000316

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:1000      

ANTIGEN INFORMATION
 
 

Antibody HPA005785

  

Antibody CAB000112

  

Antibody CAB000316

  

Antigen

 Recombinant protein fragment   Not known   Not known  

Length (aa)

 138      

Antigen sequence

 
DDVSSGSSSERSSTSGGYIFYTFSTVHPIPDEDSPWITDSTDRIPATTLM
STSATATETATKRQETWDWFSWLFLPSESKNHLHTTTQMAGTSSNTISAG
WEPNEENEDERDRHLSFSGSGIDDDEDFISSTISTTPR
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					CD44-001 - ENSP00000398632 [100%] 
CD44-028 - ENSP00000436623 [96%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CD44-001
CD44-028
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA005785

												
										 
																				
											
											
											
Antibody CAB000112

												
										 
																				
											
											
											
Antibody CAB000316

												
										 
																		

																	

										
											
1.

												
																						Expression profiling of microdissected cell populations selected from basal cells in normal epidermis and basal cell carcinoma

	Asplund A et al

	Br J Dermatol 2008;158(3):527-38

			
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						Synthesis of hyaluronan in oesophageal cancer cells is uncoupled from the prostaglandin-cAMP pathway

	Twarock S et al

	Br J Pharmacol 2009;157(2):234-43

			
											 
																						
											 
																						
											 
																				

																		

										
											
3.

												
																						Metformin regulates breast cancer stem cell ontogeny by transcriptional regulation of the epithelial-mesenchymal transition (EMT) status

	Vazquez-Martin A et al

	Cell Cycle 2010;9(18):3807-14

			
											 
																						
											 
																						
											 
																				

																		

										
											
4.

												
																						Identification of a population of blood circulating tumor cells from breast cancer patients that initiates metastasis in a xenograft assay

	Baccelli I et al

	Nat Biotechnol 2013;31(6):539-44

			
											 
																						
											 
																						
											 
																				

																		

										
											
5.

												
																						Optimization of tumor xenograft dissociation for the profiling of cell surface markers and nutrient transporters

	Petit V et al

	Lab Invest 2013;93(5):611-21

			
											 
																						
											 
																						
											 
																				

																		

										
											
6.

												
																						Superficial scrapings from breast tumors is a source for biobanking and research purposes

	Ma R et al

	Lab Invest 2014;94(7):796-805

			Application: ICC-IF				
											 
																						
											 
																						
											 
																				

																		

										
											
7.

												
																						EpCAM-Independent Enrichment of Circulating Tumor Cells in Metastatic Breast Cancer

	Schneck H et al

	PLoS One 2015;10(12):e0144535

			Application: ICC-IF				
											 
																						
											 
																						
											 
																				

																		

										
											
8.

												
																						Endocardial-to-mesenchymal transformation and mesenchymal cell colonization at the onset of human cardiac valve development

	Monaghan MG et al

	Development 2016;143(3):473-82

			Application: IHC				
											 
																						
											 
																						
											 
																				

																		

										
											
9.

												
																						Murine model: maternal administration of stem cells for prevention of prematurity

	Lei J et al

	Am J Obstet Gynecol 2015;212(5):639.e1-10

			Application: ICC-IF				
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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