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Antibody staining overlaps with antibody HPA040024.
Validated
Antibody staining overlaps with antibody HPA039530.
Standard validation
Approved
Approved
Figure description
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
Antibody dilution
1:26
1:43
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA039530
Antibody HPA040024
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 18 tissues Protein: detected in 74 cell types
RNA: detected in 18 tissues Protein: detected in 65 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:350
1:200
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA039530
Antibody HPA040024
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
57.9, 29.5, 20.9, 11, 5.4
57.9
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA039530
Antibody HPA040024
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
