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ANTIBODY INFORMATION
 
 

Antibody HPA026294

  

Antibody HPA053709

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA026294   HPA053709  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 6   13  

References

    

Proper citation

 Atlas Antibodies Cat#HPA026294, RRID:AB_10603988   Atlas Antibodies Cat#HPA053709, RRID:AB_2682237  

VALIDATION SUMMARY
 
 

Antibody HPA026294

  

Antibody HPA053709

  
Immunocytochemistry

Approved   Approved  
Immunohistochemistry

Approved   Approved  
Western blot

Supported   Uncertain  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA026294

  

Antibody HPA053709

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.   Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.  

Antibody dilution

 1:10   1:41  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA026294

  

Antibody HPA053709

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in germinal and non-germinal center cells.   Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in germinal and non-germinal center cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 73 cell types		   RNA: detected in 37 tissues
Protein: detected in 68 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:35   1:500  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA026294

  

Antibody HPA053709

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 77.8, 59.5   77.8  

Antibody dilution

 1:250   1:210  

PROTEIN ARRAY
 
 

Antibody HPA026294

  

Antibody HPA053709

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6000   1:1650  

ANTIGEN INFORMATION
 
 

Antibody HPA026294

  

Antibody HPA053709

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 61   74  

Antigen sequence

 
FKEIDWAQLNHRQIEPPFRPRIKSREDVSNFDPDFIKEEPVLTPIDEGHL
PMINQDEFRNF
  
DHFVANCTLQFQELLRTTGASDTFEGWVDLEPEGKVFVVITLTGSFTEAT
LQRDRIFKHFTRKRQRAMRRRVHQ
 

Matching transcripts

 PRKCH-001 - ENSP00000329127 [100%]
PRKCH-002 - ENSP00000450981 [100%]
  PRKCH-001 - ENSP00000329127 [100%]
 

Other gene match

    
ANTIGEN VIEW
PRKCH-001
PRKCH-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.