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Immunofluorescent staining of human cell line SH-SY5Y shows localization to plasma membrane.
Antibody dilution
1:95
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA000271
Antibody HPA001368
Antibody CAB022159
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.
Immunohistochemical staining of human bone marrow shows moderate cytoplasmic positivity in hematopoietic cells.
Expression
RNA: detected in 37 tissues Protein: detected in 48 cell types
RNA: detected in 37 tissues Protein: detected in 73 cell types
Retrieval
HIER pH9
HIER pH6
Antibody dilution
1:15
1:115
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA000271
Antibody HPA001368
Antibody CAB022159
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 207, 110, 79, 49, 32, 25, 17.1 Lane 2: RT4 Lane 3: EFO-21 Lane 4: A-431 Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 207, 110, 79, 49, 32, 25, 17.1 Lane 2: RT4 Lane 3: EFO-21 Lane 4: A-431 Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
74.5, 70
74.5, 70
74.5, 70, 14.7, 11.7
Antibody dilution
1:500
1:500
1:500
PROTEIN ARRAY
Antibody HPA000271
Antibody HPA001368
Antibody CAB022159
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
