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ANTIBODY INFORMATION
 
 

Antibody HPA002131

  

Antibody HPA063777

  

Antibody CAB002453

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Novocastra
  

Product name

 HPA002131   HPA063777   NCL-VINC  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

      

Released in version

 2   14   1  

References

      

Proper citation

 Atlas Antibodies Cat#HPA002131, RRID:AB_1080570   Atlas Antibodies Cat#HPA063777, RRID:AB_2685121    

VALIDATION SUMMARY
 
 

Antibody HPA002131

  

Antibody HPA063777

  

Antibody CAB002453

  
Immunocytochemistry

 

Validated
    
Immunohistochemistry

Supported    

Validated
  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA002131

  

Antibody HPA063777

  

Antibody CAB002453

  

Formal validation: Tagged protein

  
Validated

Antibody staining overlaps with GFP tagged protein		    

     

Figure description

   Immunofluorescent staining of transgenic HeLa cells show antibody staining in focal adhesion sites and GFP expression in focal adhesion sites.    

Antibody dilution

   1:130    
 

Standard validation

   Supported    

     

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to focal adhesion sites.    

Antibody dilution

   1:130    

Literature conformity

   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002131

  

Antibody HPA063777

  

Antibody CAB002453

  

Formal validation: Orthogonal

    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

   
 

Figure description

     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.  
 

Standard validation

 Supported     Supported  

     

Figure description

 Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.     Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 65 cell types		     RNA: detected in 37 tissues
Protein: detected in 60 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:250     1:400  

Literature conformity

 Consistent with extensive gene/protein characterization data.     Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002131

  

Antibody HPA063777

  

Antibody CAB002453

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 123.8, 116.7   123.8, 116.7, 87.4   123.8, 116.7, 87.4  

Antibody dilution

 1:500   1:430   1:500  

PROTEIN ARRAY
 
 

Antibody HPA002131

  

Antibody HPA063777

  

Antibody CAB002453

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA002131

  

Antibody HPA063777

  

Antibody CAB002453

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 149   98    

Antigen sequence

 
KMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQG
IEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALAS
IDSKLNQAKGWLRDPSASPGDAGEQAIRQILDEAGKVGELCAGKERREI
  
SALTSKLADLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAK
AAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGP
  

											 
																				

																		

										
											
Matching transcripts

												
																					VCL-001 - ENSP00000361841 [100%] 
VCL-201 - ENSP00000211998 [100%] 

											 
																						VCL-001 - ENSP00000361841 [100%] 
VCL-002 - ENSP00000415489 [100%] 
VCL-201 - ENSP00000211998 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

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