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CDH1

ANTIBODY INFORMATION
 
 

Antibody HPA004812

  

Antibody CAB000087

  

Antibody CAB028364

  

Antibody CAB072855

  

Antibody CAB072856

  

Antibody CAB072857

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Zymed
   ECM Biosciences
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA004812   13-1700   CM1681   AMAb90862   AMAb90863   AMAb90865  

Host species

 Rabbit   Mouse   Mouse   Mouse   Mouse   Mouse  

Clonality

 pAb   mAb   mAb   mAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known   Protein A/G   Protein A/G   Protein A/G   Protein A/G  

Other gene match

            

Released in version

 3   1   6   14   14   14  

References

            

Proper citation

 Atlas Antibodies Cat#HPA004812, RRID:AB_1078369     ECM Biosciences Cat#CM1681, RRID:AB_2076812   Atlas Antibodies Cat#AMAb90862, RRID:AB_2665698   Atlas Antibodies Cat#AMAb90863, RRID:AB_2665699   Atlas Antibodies Cat#AMAb90865, RRID:AB_2665700  

VALIDATION SUMMARY
 
 

Antibody HPA004812

  

Antibody CAB000087

  

Antibody CAB028364

  

Antibody CAB072855

  

Antibody CAB072856

  

Antibody CAB072857

  
Immunocytochemistry

    Supported        
Immunohistochemistry


Validated
  

Validated
  

Validated
  

Validated
  

Validated
  

Validated
  
Western blot

Uncertain   Supported   Uncertain   Supported   Supported   Supported  
Protein array

Supported            

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA004812

  

Antibody CAB000087

  

Antibody CAB028364

  

Antibody CAB072855

  

Antibody CAB072856

  

Antibody CAB072857

  

Standard validation

     Supported        

           

Figure description

     Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, the Golgi apparatus & cell junctions.        

Antibody dilution

     1:112        

Literature conformity

     The subcellular location is supported by literature.        

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA004812

  

Antibody CAB000087

  

Antibody CAB028364

  

Antibody CAB072855

  

Antibody CAB072856

  

Antibody CAB072857

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

   
 
 
 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 
 
 
 
 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

 Supported   Supported   Supported   Supported   Supported   Supported  

           

Figure description

 Immunohistochemical staining of human kidney shows membranous positivity in distal tubules.   Immunohistochemical staining of human duodenum shows strong membranous positivity in glandular cells.   Immunohistochemical staining of human prostate shows strong membranous and cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human stomach shows membranous positivity in glandular cells.   Immunohistochemical staining of human esophagus shows strong membranous positivity in squamous epithelial cells.   Immunohistochemical staining of human stomach shows membranous positivity in glandular cells.  

Expression

 RNA: detected in 33 tissues
Protein: detected in 41 cell types		   RNA: detected in 33 tissues
Protein: detected in 37 cell types		   RNA: detected in 33 tissues
Protein: detected in 43 cell types		   RNA: detected in 33 tissues
Protein: detected in 40 cell types		   RNA: detected in 33 tissues
Protein: detected in 39 cell types		   RNA: detected in 33 tissues
Protein: detected in 36 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:300   1:10000   1:450   1:750   1:1000   1:850  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA004812

  

Antibody CAB000087

  

Antibody CAB028364

  

Antibody CAB072855

  

Antibody CAB072856

  

Antibody CAB072857

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

           

Figure description

 Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 100, 97.5, 90.9   100, 97.5, 90.9, 71.4, 71.3   100, 97.5, 90.9, 71.4, 71.3   100, 97.5, 90.9, 71.4, 71.3   100, 97.5, 90.9, 71.4, 71.3   100, 97.5, 90.9, 71.4, 71.3  

Antibody dilution

 1:250   1:500   1:500   1:500   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA004812

  

Antibody CAB000087

  

Antibody CAB028364

  

Antibody CAB072855

  

Antibody CAB072856

  

Antibody CAB072857

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		            

           

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.            

Antibody dilution

 1:3000            

ANTIGEN INFORMATION
 
 

Antibody HPA004812

  

Antibody CAB000087

  

Antibody CAB028364

  

Antibody CAB072855

  

Antibody CAB072856

  

Antibody CAB072857

  

Antigen

 Recombinant protein fragment   Not known   Recombinant protein   Recombinant protein   Recombinant protein   Recombinant protein  

Length (aa)

 125            

Antigen sequence

 
ATDNGSPVATGTGTLLLILSDVNDNAPIPEPRTIFFCERNPKPQVINIID
ADLPPNTSPFTAELTHGASANWTIQYNDPTQESIILKPKMALEVGDYKIN
LKLMDNQNKDQVTTLEVSVCDCEGA
  

											 
																						

											 
																						

											 
																						

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					CDH1-001 - ENSP00000261769 [100%] 
CDH1-004 - ENSP00000414946 [100%] 
CDH1-201 - ENSP00000481063 [100%] 

											 
																						
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CDH1-001
CDH1-004
CDH1-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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