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Immunohistochemical staining of human kidney shows membranous positivity in distal tubules.
Immunohistochemical staining of human duodenum shows strong membranous positivity in glandular cells.
Immunohistochemical staining of human prostate shows strong membranous and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach shows membranous positivity in glandular cells.
Immunohistochemical staining of human esophagus shows strong membranous positivity in squamous epithelial cells.
Immunohistochemical staining of human stomach shows membranous positivity in glandular cells.
Expression
RNA: detected in 33 tissues Protein: detected in 41 cell types
RNA: detected in 33 tissues Protein: detected in 37 cell types
RNA: detected in 33 tissues Protein: detected in 43 cell types
RNA: detected in 33 tissues Protein: detected in 40 cell types
RNA: detected in 33 tissues Protein: detected in 39 cell types
RNA: detected in 33 tissues Protein: detected in 36 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:300
1:10000
1:450
1:750
1:1000
1:850
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA004812
Antibody CAB000087
Antibody CAB028364
Antibody CAB072855
Antibody CAB072856
Antibody CAB072857
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
100, 97.5, 90.9
100, 97.5, 90.9, 71.4, 71.3
100, 97.5, 90.9, 71.4, 71.3
100, 97.5, 90.9, 71.4, 71.3
100, 97.5, 90.9, 71.4, 71.3
100, 97.5, 90.9, 71.4, 71.3
Antibody dilution
1:250
1:500
1:500
1:500
1:500
1:500
PROTEIN ARRAY
Antibody HPA004812
Antibody CAB000087
Antibody CAB028364
Antibody CAB072855
Antibody CAB072856
Antibody CAB072857
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
