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DNAH5

ANTIBODY INFORMATION
 
 

Antibody HPA037469

  

Antibody HPA037470

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA037469   HPA037470  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   7  

References

   3  

Proper citation

 Atlas Antibodies Cat#HPA037469, RRID:AB_10672791   Atlas Antibodies Cat#HPA037470, RRID:AB_10672348  

VALIDATION SUMMARY
 
 

Antibody HPA037469

  

Antibody HPA037470

  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Approved   Approved  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA037469

  

Antibody HPA037470

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human fallopian tube shows distinct positivity in the cilia.   Immunohistochemical staining of human nasopharynx shows strong positivity in the cilia of respiratory epithelial cells.  

Expression

 RNA: detected in 6 tissues
Protein: detected in 14 cell types		   RNA: detected in 6 tissues
Protein: detected in 23 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:15   1:750  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA037469

  

Antibody HPA037470

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 529   529  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA037469

  

Antibody HPA037470

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA037469

  

Antibody HPA037470

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 88   89  

Antigen sequence

 
NFDTLTSSINARANALLLTTVTRKKKETEMLGEEARELLSHFNHQNMDAL
LKVTRNTLEAIRKRIHSSHTINFRDSNSASNMKQNSLP
  
EVEDAILEGNQIERIDQLFAVGGLRHLMFYYQDVEEAETGQLGSLGGVNL
VSGKIKKPKVFVTEGNDVALTGVCVFFIRTDPSKAITPD
 

Matching transcripts

 DNAH5-001 - ENSP00000265104 [100%]
  DNAH5-001 - ENSP00000265104 [100%]
 

Other gene match

    
ANTIGEN VIEW
DNAH5-001

RELEVANT PUBLICATIONS
 
 

Antibody HPA037469

  

Antibody HPA037470

  

1.

   Combined exome and whole-genome sequencing identifies mutations in ARMC4 as a cause of primary ciliary dyskinesia with defects in the outer dynein arm
Onoufriadis A et al
J Med Genet 2014;51(1):61-7
  

2.

   Targeted NGS gene panel identifies mutations in RSPH1 causing primary ciliary dyskinesia and a common mechanism for ciliary central pair agenesis due to radial spoke defects
Onoufriadis A et al
Hum Mol Genet 2014;23(13):3362-74
  

3.

   HEATR2 plays a conserved role in assembly of the ciliary motile apparatus
Diggle CP et al
PLoS Genet 2014;10(9):e1004577
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.