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ANTIBODY INFORMATION
 
 

Antibody HPA019661

  

Antibody HPA019663

  

Antibody HPA024336

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA019661   HPA019663   HPA024336  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 5   5   5  

References

      

Proper citation

 Atlas Antibodies Cat#HPA019661, RRID:AB_1858215   Atlas Antibodies Cat#HPA019663, RRID:AB_1858216   Atlas Antibodies Cat#HPA024336, RRID:AB_1858217  

VALIDATION SUMMARY
 
 

Antibody HPA019661

  

Antibody HPA019663

  

Antibody HPA024336

  
Immunocytochemistry


Validated
  

Validated
  

Validated
  
Immunohistochemistry

Supported   Supported   Supported  
Western blot


Validated
   Uncertain   Uncertain  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA019661

  

Antibody HPA019663

  

Antibody HPA024336

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA024336.
Antibody staining overlaps with antibody HPA019663.		  
Validated

Antibody staining overlaps with antibody HPA024336.
Antibody staining overlaps with antibody HPA019661.		  
Validated

Antibody staining overlaps with antibody HPA019663.
Antibody staining overlaps with antibody HPA019661.		  
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nuclear membrane.   Immunofluorescent staining of human cell line A-431 shows localization to nuclear membrane.   Immunofluorescent staining of human cell line A-431 shows localization to nuclear membrane.  

Antibody dilution

 1:50   1:7   1:10  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA019661

  

Antibody HPA019663

  

Antibody HPA024336

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human hippocampus shows distinct nuclear membrane positivity in neuronal cells.   Immunohistochemical staining of human urinary bladder shows distinct nuclear positivity in urothelial cells.   Immunohistochemical staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 74 cell types		   RNA: detected in 37 tissues
Protein: detected in 59 cell types		   RNA: detected in 37 tissues
Protein: detected in 81 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:20   1:20  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA019661

  

Antibody HPA019663

  

Antibody HPA024336

  

Formal validation: Genetic
Validated

Downregulation visible in one of two siRNA lanes		      

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		      

Target mass (kDa)

 267.3      

Loading control

 Total protein image      

Antibody dilution

 1:111      
 

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		  

     

Figure description

      

Target mass (kDa)

 267.3   267.3, 84   267.3  

Antibody dilution

 1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA019661

  

Antibody HPA019663

  

Antibody HPA024336

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:500   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA019661

  

Antibody HPA019663

  

Antibody HPA024336

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 90   140   106  

Antigen sequence

 
KMASVRQHLEETTQKAESQLLECKASWEERERMLKDEVSKCVCRCEDLEK
QNRLLHDQIEKLSDKVVASVKEGVQGPLNVSLSEEGKSQE
  
RDEEVSSADISSSSEVISQHLVSYRNIEELQQQNQRLLVALRELGETRER
EEQETTSSKITELQLKLESALTELEQLRKSRQHQMQLVDSIVRQRDMYRI
LLSQTTGVAIPLHASSLDDVSLASTPKRPSTSQTVSTPAP
  
RRPPHPLPPRLTIHAPPQELGPPVQRIQMTRRQSVGRGLQLTPGIGGMQQ
HFFDDEDRTVPSTPTLVVPHRTDGFAEAIHSPQVAGVPRFRFGPPEDMPQ
TSSSHS
 

Matching transcripts

 TPR-001 - ENSP00000356448 [100%]
  TPR-001 - ENSP00000356448 [100%]
TPR-201 - ENSP00000483425 [100%]
  TPR-001 - ENSP00000356448 [100%]
 

Other gene match

      
ANTIGEN VIEW
TPR-001
TPR-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.