BCAR1 - Antibodies - The Human Protein Atlas

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BCAR1

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA042282

Antibody CAB000443

Provider

Atlas Antibodies
Sigma-Aldrich

Lab Vision/NeoMarkers

Product name

HPA042282

MS-855

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Not known

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA042282, RRID:AB_2677929

VALIDATION SUMMARY

Antibody HPA042282

Antibody CAB000443

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA042282

Antibody CAB000443

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.

Antibody dilution

1:200

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA042282

Antibody CAB000443

Standard validation

Approved

Figure description

Immunohistochemical staining of human spleen shows strong cytoplasmic positivity.

Expression

RNA: detected in 36 tissues
Protein: detected in 44 cell types

RNA: detected in 36 tissues
Protein: detected in 48 cell types

Retrieval

HIER pH6

Antibody dilution

1:250

1:1600

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

Mainly not consistent with RNA expression data.

Standard validation

Supported

Figure description

labelling of neurons throuhtout the brain, strongest immunoreactiivity in cerebellum and olfactory bulb.

WESTERN BLOT

Antibody HPA042282

Antibody CAB000443

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

97.9, 95.5, 95.2, 93.4, 93.3, 93.1, 25.5

97.9, 95.5, 95.2, 93.4, 93.3, 93.1, 77.7, 25.5, 14.4, 14.2, 13.8, 12.8, 8.5, 6.2, 5.7, 5.4

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA042282

Antibody CAB000443

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:6000

ANTIGEN INFORMATION

Antibody HPA042282

Antibody CAB000443

Antigen

Recombinant protein fragment

Not known

Length (aa)

102

Antigen sequence

SPQFQSPPAKQTSTFSKQTPHHPFPSPATDLYQVPPGPGGPAQDIYQVPP
SAGMGHDIYQVPPSMDTRSWEGTKPPAKVVVPTRVGQGYVYEAAQPEQDE
YD

Matching transcripts

BCAR1-001 - ENSP00000377074 [100%]
BCAR1-002 - ENSP00000162330 [100%]
BCAR1-003 - ENSP00000392708 [100%]
BCAR1-004 - ENSP00000443841 [100%]
BCAR1-005 - ENSP00000391669 [100%]
BCAR1-008 - ENSP00000377072 [100%]
BCAR1-009 - ENSP00000440415 [100%]
BCAR1-017 - ENSP00000454702 [99%]

Other gene match

ANTIGEN VIEW

BCAR1-001	BCAR1-002	BCAR1-003	BCAR1-004	BCAR1-005	BCAR1-008	BCAR1-009	BCAR1-017

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.