Cell atlas - THOC3 - The Human Protein Atlas

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THOC3

TISSUE

CELL

PATHOLOGY

CELL ATLAS RNA EXPRESSION HUMAN CELLS CUSTOM DATA MOUSE CELLS

GENE/PROTEIN

Antibody validation

Dictionary
	Nucleoplasm

Human cell
	Nucleoplasm

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GENERAL INFORMATION
Gene name	THOC3
Gene description	THO complex 3
Protein class	Predicted intracellular proteins Predicted secreted proteins
Predicted localization	Intracellular,Secreted
Number of transcripts	7
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HUMAN PROTEIN ATLAS INFORMATION
Summary	Mainly localized to the nucleoplasm.
RNA cell category	Expressed in all
Protein evidence	Evidence at protein level
Main location	Localized to the Nucleoplasm (validated)
Single-cell variation	Single-cell variation in protein expression observed.
Cell cycle dependency	Cell cycle dependent expression determined by custom assay
Mouse	Localized to the Nucleoplasm in mouse cells.

DATA RELIABILITY
Reliability score	Validated based on 2 antibodies. HPA042764 , HPA045071
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ASSAYS

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all Organ Origin Category Expression Alphabetical Cell lines sorted after organ of phenotypic resemblance. Cell lines sorted after biological source for establishment. Cell lines sorted after the cell line category according to Cellosaurus. Cell lines sorted on descending RNA expression. Cell lines sorted alphabetically.

HUMAN CELLS

Summary

Mainly localized to the nucleoplasm.

Main location

Nucleoplasm (validated)


	Toggle channels	Low High G1 S G2 M N/A

Antibody	Cell line	Location	Single-cell variation	Cell cycle dependent variation
HPA042764	A-431	Nucleoplasm
HPA042764	A-431	Vesicles
HPA042764	U-2 OS	Nucleoplasm
HPA042764	U-2 OS	Vesicles
HPA042764	U-251 MG	Nucleoplasm
HPA042764	U-251 MG	Vesicles
HPA045071	A-431	Nucleoplasm
HPA045071	U-2 OS	Nucleoplasm	Intensity	Intensity
HPA045071	U-251 MG	No staining

CUSTOM DATA


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Thumbnail	Antibody	Cell line	Assay	Description
	HPA045071	U-2 OS	Image	Staining of U-2 OS FUCCI cells, to characterize the cell cycle dependency of the protein expression pattern. The FUCCI cells, Fluorescence Ubiquitination Cell Cycle Indicator, are cells tagged with different fluorescent proteins fused to two different cell cycle regulators Cdt1 (expressed in G1 phase) and Geminin (expressed in S and G2 phases) that allows cell cycle monitoring. When both proteins are present, the overlay of the images appear in yellow marking the G1/S transition. THOC3 is mostly expressed at the G1/S checkpoint, and during S and/or G2.

CELL CYCLE
Summary	Expression has cell cycle dependency according to custom assay
Intensity variation	Nucleoplasm

MOUSE CELLS

Main location

Nucleoplasm

Additional location

Vesicles

Mouse gene

ENSMUSG00000025872 (version 83)


	Toggle channels	Low High G1 S G2 M N/A

Antibody	Cell line	Location
HPA042764	NIH 3T3	Nucleoplasm
HPA045071	NIH 3T3	Nucleoplasm
HPA045071	NIH 3T3	Vesicles

GENE INFORMATION
Gene name	THOC3 (HGNC Symbol)
Synonyms	MGC5469, TEX1
Description	THO complex 3 (HGNC Symbol)
Entrez gene summary	This gene encodes a component of the nuclear THO transcription elongation complex, which is part of the larger transcription export (TREX) complex that couples messenger RNA processing and export. In humans, the transcription export complex is recruited to the 5'-end of messenger RNAs in a splicing- and cap-dependent manner. Studies of a related complex in mouse suggest that the metazoan transcription export complex is involved in cell differentiation and development. A pseudogene of this gene has been defined on chromosome 5. [provided by RefSeq, May 2013]
Chromosome	5
Cytoband	q35.2
Chromosome location (bp)	175917873 - 176034680
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000051596 (version 83.38)
Entrez gene	84321
UniProt	Q96J01 (UniProt - Evidence at protein level)
neXtProt	NX_Q96J01
Antibodypedia	THOC3 antibodies

PROTEIN BROWSER

THOC3-001	THOC3-002	THOC3-003	THOC3-004	THOC3-008	THOC3-010	THOC3-201

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
THOC3-001 ENSP00000265097 ENST00000265097	Q96J01 [Direct mapping] THO complex subunit 3 Show all	Predicted intracellular proteins Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000346 [transcription export complex] GO:0000445 [THO complex part of transcription export complex] GO:0003723 [RNA binding] GO:0005515 [protein binding] GO:0005654 [nucleoplasm] GO:0005794 [Golgi apparatus] GO:0006397 [mRNA processing] GO:0006406 [mRNA export from nucleus] GO:0008380 [RNA splicing] GO:0016607 [nuclear speck] GO:0043231 [intracellular membrane-bounded organelle] GO:0046784 [viral mRNA export from host cell nucleus] Show all	351 aa 38.8 kDa	No	0
THOC3-002 ENSP00000425781 ENST00000515016	H0YA11 [Direct mapping] THO complex subunit 3 Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all		57 aa 6.1 kDa	No	0
THOC3-003 ENSP00000394166 ENST00000432305	H7C0C5 [Direct mapping] THO complex subunit 3 Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0005515 [protein binding] Show all	75 aa 8.4 kDa	No	0
THOC3-004 ENSP00000422243 ENST00000513482	Q96J01 [Direct mapping] THO complex subunit 3 Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0000346 [transcription export complex] GO:0000445 [THO complex part of transcription export complex] GO:0003723 [RNA binding] GO:0005515 [protein binding] GO:0005654 [nucleoplasm] GO:0005794 [Golgi apparatus] GO:0006397 [mRNA processing] GO:0006406 [mRNA export from nucleus] GO:0008380 [RNA splicing] GO:0016607 [nuclear speck] GO:0043231 [intracellular membrane-bounded organelle] GO:0046784 [viral mRNA export from host cell nucleus] Show all	327 aa 36 kDa	No	0
THOC3-008 ENSP00000424967 ENST00000505969	H0Y9T1 [Direct mapping] THO complex subunit 3 Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0005515 [protein binding] Show all	56 aa 5.9 kDa	No	0
THOC3-010 ENSP00000425039 ENST00000514861	D6REC9 [Direct mapping] THO complex subunit 3 Show all	Predicted secreted proteins Secreted proteins predicted by MDSEC SignalP predicted secreted proteins Phobius predicted secreted proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0005515 [protein binding] GO:0005654 [nucleoplasm] GO:0005794 [Golgi apparatus] Show all	166 aa 18.4 kDa	Yes	0
THOC3-201 ENSP00000487428 ENST00000628318	D6RGZ2 [Direct mapping] THO complex subunit 3 Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0005515 [protein binding] Show all	105 aa 11.1 kDa	No	0

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.