ARID4B - Antibodies - The Human Protein Atlas

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ARID4B

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA027333

Antibody HPA053692

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA027333

HPA053692

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA027333, RRID:AB_1845013

VALIDATION SUMMARY

Antibody HPA027333

Antibody HPA053692

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA027333

Antibody HPA053692

Standard validation

Uncertain

Figure description

Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm & vesicles.

Antibody dilution

1:26

Literature conformity

The subcellular location is not consistent with literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA027333

Antibody HPA053692

Standard validation

Approved

Figure description

Immunohistochemical staining of human placenta shows moderate nuclear positivity in decidual cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 44 cell types

Retrieval

HIER pH6

Antibody dilution

1:75

Literature conformity

Partly consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA027333

Antibody HPA053692

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

147.8, 137.6, 61.4

Antibody dilution

1:250

1:130

PROTEIN ARRAY

Antibody HPA027333

Antibody HPA053692

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

1:1050

ANTIGEN INFORMATION

Antibody HPA027333

Antibody HPA053692

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

DNNGKEESKIDHLTNNRNDLISKEEQNSSSLLEENKVHADLVISKPVSKS
PERLRKDIEVLSEDTDYEEDEVTKKRKDVKKDTTDKSSKPQIKRGKR

VAEEESCSPSVELEKPPPVNVDSKPIEEKTVEVNDRKAEFPSSGSNSVLN
TPPTTPESPSSVTVTEGSRQQSSVTVSEPLAPN

Matching transcripts

ARID4B-001 - ENSP00000264184 [100%]
ARID4B-004 - ENSP00000355562 [100%]
ARID4B-006 - ENSP00000416063 [100%]
ARID4B-201 - ENSP00000264183 [100%]

Other gene match

ANTIGEN VIEW

ARID4B-001	ARID4B-004	ARID4B-006	ARID4B-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.