We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to cytosol.
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Antibody dilution
1:145
1:50
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA019795
Antibody HPA063893
Antibody CAB003845
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human cerebral cortex shows cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Expression
RNA: detected in 37 tissues Protein: detected in 22 cell types
RNA: detected in 37 tissues Protein: detected in 54 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:7
1:200
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA019795
Antibody HPA063893
Antibody CAB003845
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6 Lane 2: RT4 Lane 3: U-251 MG Lane 4: A-431 Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
62.1, 57.4
62.1, 57.4
62.1, 57.4, 15.7, 11.3
Antibody dilution
1:250
1:480
1:500
PROTEIN ARRAY
Antibody HPA019795
Antibody HPA063893
Antibody CAB003845
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
