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Antibody staining overlaps with antibody HPA052860.
Validated
Antibody staining overlaps with antibody HPA012082.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & nuclear speckles.
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli & nuclear speckles.
Antibody dilution
1:26
1:10
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA012082
Antibody HPA012395
Antibody HPA052860
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human liver shows strong nuclear positivity in hepatocytes.
Expression
RNA: detected in 37 tissues Protein: detected in 28 cell types
Retrieval
HIER pH6
Antibody dilution
1:25
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Standard validation
Approved
Figure description
Observed immunoreactivity in neuronal cells throughout the brain.
WESTERN BLOT
Antibody HPA012082
Antibody HPA012395
Antibody HPA052860
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
122.9, 60.3
122.9, 60.3
122.9
Antibody dilution
1:250
1:250
1:100
PROTEIN ARRAY
Antibody HPA012082
Antibody HPA012395
Antibody HPA052860
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
