LAMC2 - Antibodies - The Human Protein Atlas

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LAMC2

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA024638

Antibody CAB004257

Antibody CAB078165

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Atlas Antibodies
Sigma-Aldrich

Product name

HPA024638

sc-25341

AMAb91098

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA024638, RRID:AB_1852689

Santa Cruz Biotechnology Cat#sc-25341, RRID:AB_627873

Atlas Antibodies Cat#AMAb91098, RRID:AB_2665800

VALIDATION SUMMARY

Antibody HPA024638

Antibody CAB004257

Antibody CAB078165

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA024638

Antibody CAB004257

Antibody CAB078165

Standard validation

Uncertain

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to cytosol.

Antibody dilution

1:200

Literature conformity

The subcellular location is not consistent with literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA024638

Antibody CAB004257

Antibody CAB078165

Standard validation

Approved

Figure description

Immunohistochemical staining of human oral mucosa shows distinct positivity in basement membrane of squamous epithelia.

Immunohistochemical staining of human vagina shows distinct positivity in the basement membrane of squamous epithelial cells.

Immunohistochemical staining of human fallopian tube shows strong positivity in basal membrane of glandular cells.

Expression

RNA: detected in 29 tissues
Protein: detected in 36 cell types

RNA: detected in 29 tissues
Protein: detected in 56 cell types

RNA: detected in 29 tissues
Protein: detected in 14 cell types

Retrieval

HIER pH6

Antibody dilution

1:900

1:50

1:1300

Literature conformity

Partly consistent with extensive gene/protein characterization data.

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA024638

Antibody CAB004257

Antibody CAB078165

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Testis

Target mass (kDa)

131

131, 121.6

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA024638

Antibody CAB004257

Antibody CAB078165

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA024638

Antibody CAB004257

Antibody CAB078165

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

Antigen sequence

NAGVTIQDTLNTLDGLLHLMDQPLSVDEEGLVLLEQKLSRAKTQINSQLR
PMMSELEERARQQRGHLHLLETSIDGILADVKNLEN

Matching transcripts

LAMC2-001 - ENSP00000264144 [100%]

Other gene match

ANTIGEN VIEW

LAMC2-001

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.