Fields »
ABOUT
HELP
BLOG
SLC2A3

ANTIBODY INFORMATION
 
 

Antibody HPA006539

  

Antibody CAB002763

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Chemicon
  

Product name

 HPA006539   AB1345  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

 SLC2A14 - ENSG00000173262 [98%]
    

Released in version

 3   1  

References

    

Proper citation

 Atlas Antibodies Cat#HPA006539, RRID:AB_1078984    

VALIDATION SUMMARY
 
 

Antibody HPA006539

  

Antibody CAB002763

  
Immunocytochemistry

Approved    
Immunohistochemistry

Approved   Approved  
Western blot

Supported   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA006539

  

Antibody CAB002763

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line CACO-2 shows localization to plasma membrane.    

Antibody dilution

 1:10    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA006539

  

Antibody CAB002763

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.   Immunohistochemical staining of human testis shows strong cytoplasmic and membranous positivity in cells in seminiferus ducts.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 25 cell types		   RNA: detected in 36 tissues
Protein: detected in 35 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:100   1:2500  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA006539

  

Antibody CAB002763

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 58.2, 56.3, 54, 53.9, 44.9, 17.4   53.9, 15  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA006539

  

Antibody CAB002763

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA006539

  

Antibody CAB002763

  

Antigen

 Recombinant protein fragment   Not known  

Length (aa)

 46    

Antigen sequence

 
KVPETRGRTFEDITRAFEGQAHGADRSGKDGVMEMNSIEPAKETTT
   

Matching transcripts

 SLC2A3-001 - ENSP00000075120 [100%]
   

Other gene match

 SLC2A14 - ENSG00000173262 [98%]
    
ANTIGEN VIEW
SLC2A3-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.