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CASP8

ANTIBODY INFORMATION
 
 

Antibody HPA001302

  

Antibody HPA005688

  

Antibody HPA006191

  

Antibody CAB002047

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Novocastra
  

Product name

 HPA001302   HPA005688   HPA006191   NCL-CASP-8  

Host species

 Rabbit   Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

        

Released in version

 4   4   12   1  

References

        

Proper citation

 Atlas Antibodies Cat#HPA001302, RRID:AB_1846057   Atlas Antibodies Cat#HPA005688, RRID:AB_1846056   Atlas Antibodies Cat#HPA006191, RRID:AB_2667535    

VALIDATION SUMMARY
 
 

Antibody HPA001302

  

Antibody HPA005688

  

Antibody HPA006191

  

Antibody CAB002047

  
Immunocytochemistry

Supported     Supported    
Immunohistochemistry

Approved   Approved     Approved  
Western blot

Supported   Supported   Uncertain   Supported  
Protein array

Approved   Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001302

  

Antibody HPA005688

  

Antibody HPA006191

  

Antibody CAB002047

  

Standard validation

 Supported     Supported    

       

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.     Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.    

Antibody dilution

 1:143     1:38    

Literature conformity

 The subcellular location is supported by literature.     The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001302

  

Antibody HPA005688

  

Antibody HPA006191

  

Antibody CAB002047

  

Standard validation

 Approved   Approved     Approved  

       

Figure description

 Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in germinal center and non-germinal center cells.   Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in germinal center cells and non-germinal center cells.     Immunohistochemical staining of human duodenum shows strong positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 66 cell types		   RNA: detected in 37 tissues
Protein: detected in 78 cell types		     RNA: detected in 37 tissues
Protein: detected in 52 cell types		  

Retrieval

 HIER pH6   HIER pH6     HIER pH6  

Antibody dilution

 1:450   1:65     1:60  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.     Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.     Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001302

  

Antibody HPA005688

  

Antibody HPA006191

  

Antibody CAB002047

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

       

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 229, 107, 78, 46, 31, 26, 16.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 61.8, 57.7, 55.4, 53.8, 45.9, 29.5   61.8, 57.7, 55.4, 53.8   45.9, 29.5   61.8, 57.7, 55.4, 53.8, 45.9, 32.5, 29.5, 27.5, 24.7, 23.5, 17.3, 14.9, 13.9, 9.4  

Antibody dilution

 1:250   1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA001302

  

Antibody HPA005688

  

Antibody HPA006191

  

Antibody CAB002047

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:2000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA001302

  

Antibody HPA005688

  

Antibody HPA006191

  

Antibody CAB002047

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 146   147   125    

Antigen sequence

 
GIIYGTDGQEAPIYELTSQFTGLKCPSLAGKPKVFFIQACQGDNYQKGIP
VETDSEEQPYLEMDLSSPQTRYIPDEADFLLGMATVNNCVSYRNPAEGTW
YIQSLCQSLRERCPRGDDILTILTEVNYEVSNKDDKKNMGKQMPQP
  
LGEGKLDILKRVCAQINKSLLKIINDYEEFSKGEELCGVMTISDSPREQD
SESQTLDKVYQMKSKPRGYCLIINNHNFAKAREKVPKLHSIRDRNGTHLD
AGALTTTFEELHFEIKPHDDCTVEQIYEILKIYQLMDHSNMDCFICC
  
MEKRVILGEGKLDILKRVCAQINKSLLKIINDYEEFSKGALTTTFEELHF
EIKPHDDCTVEQIYEILKIYQLMDHSNMDCFICCILSHGDKGIIYGTDGQ
EAPIYELTSQFTGLKCPSLAGKPKV
  

											 
																				

																		

										
											
Matching transcripts

												
																					CASP8-002 - ENSP00000351273 [100%] 
CASP8-006 - ENSP00000264275 [100%] 
CASP8-009 - ENSP00000376091 [100%] 
CASP8-012 - ENSP00000325722 [100%] 
CASP8-014 - ENSP00000412523 [100%] 
CASP8-017 - ENSP00000394434 [100%] 
CASP8-201 - ENSP00000264274 [100%] 

											 
																						CASP8-006 - ENSP00000264275 [100%] 
CASP8-009 - ENSP00000376091 [100%] 
CASP8-012 - ENSP00000325722 [100%] 
CASP8-002 - ENSP00000351273 [91%] 
CASP8-014 - ENSP00000412523 [91%] 

											 
																						CASP8-017 - ENSP00000394434 [100%] 
CASP8-201 - ENSP00000264274 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CASP8-002
CASP8-006
CASP8-009
CASP8-012
CASP8-014
CASP8-017
CASP8-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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