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NGFR

ANTIBODY INFORMATION
 
 

Antibody HPA004765

  

Antibody CAB000143

  

Antibody CAB001995

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   DakoCytomation
   Upstate
  

Product name

 HPA004765   M3507   05-446  

Host species

 Rabbit   Mouse   Mouse  

Clonality

 pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Supernatant   Not known  

Other gene match

      

Released in version

 3   1   1  

References

 2      

Proper citation

 Atlas Antibodies Cat#HPA004765, RRID:AB_1079233      

VALIDATION SUMMARY
 
 

Antibody HPA004765

  

Antibody CAB000143

  

Antibody CAB001995

  
Immunocytochemistry

Approved      
Immunohistochemistry


Validated
  

Validated
  

Validated
  
Western blot

Supported   Uncertain   Uncertain  
Protein array

Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA004765

  

Antibody CAB000143

  

Antibody CAB001995

  

Standard validation

 Approved      

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.      

Antibody dilution

 1:125      

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA004765

  

Antibody CAB000143

  

Antibody CAB001995

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in reaction center cells.   Immunohistochemical staining of human vagina shows distinct cytoplasmic and membranous positivity in basal cells of squamous epithelial cells.   Immunohistochemical staining of human vagina shows distinct cytoplasmic and membranous positivity in basal cells of squamous epithelial cells.  

Expression

 RNA: detected in 31 tissues
Protein: detected in 65 cell types		   RNA: detected in 31 tissues
Protein: detected in 20 cell types		   RNA: detected in 31 tissues
Protein: detected in 13 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:500   1:50   1:200  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA004765

  

Antibody CAB000143

  

Antibody CAB001995

  

Standard validation

 Supported

Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419286)		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 45.2, 35.8   45.2, 35.8, 0.4   45.2, 35.8, 0.4  

Antibody dilution

 1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA004765

  

Antibody CAB000143

  

Antibody CAB001995

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000      

ANTIGEN INFORMATION
 
 

Antibody HPA004765

  

Antibody CAB000143

  

Antibody CAB001995

  

Antigen

 Recombinant protein fragment   Not known   Not known  

Length (aa)

 141      

Antigen sequence

 
NKQGANSRPVNQTPPPEGEKLHSDSGISVDSQSLHDQQPHTQTASGQALK
GDGGLYSSLPPAKREEVEKLLNGSAGDTWRHLAGELGYQPEHIDSFTHEA
CPVRALLASWATQDSATLDALLAALRRIQRADLVESLCSES
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					NGFR-001 - ENSP00000172229 [100%] 
NGFR-002 - ENSP00000421731 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
NGFR-001
NGFR-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA004765

												
										 
																				
											
											
											
Antibody CAB000143

												
										 
																				
											
											
											
Antibody CAB001995

												
										 
																		

																	

										
											
1.

												
																						p75 Neurotrophin receptor differentiates between morphoeic basal cell carcinoma and desmoplastic trichoepithelioma: insights into the histogenesis of adnexal tumours based on embryology and hair follicle biology

	Krahl D et al

	Br J Dermatol 2010;163(1):138-45

			
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						Stem cells from human exfoliated deciduous tooth exhibit stromal-derived inducing activity and lead to generation of neural crest cells from human embryonic stem cells

	Karbalaie K et al

	Cell J 2015;17(1):37-48

			Application: ICC-IF				
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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