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Immunohistochemical staining of human stomach shows strong granular positivity in cytoplasm of glandular cells.
Immunohistochemical staining of human testis shows moderate, granular cytoplasmic and nuclear positivity in cells in seminiferous ducts and Leydig cells.
Expression
RNA: detected in 37 tissues Protein: detected in 66 cell types
RNA: detected in 37 tissues Protein: detected in 59 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:250
1:75
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Mainly consistent with RNA expression data.
Standard validation
Supported
Figure description
Cytoplasmic staining with a granular pattern was observed in subsets of neurons.
WESTERN BLOT
Antibody HPA000992
Antibody HPA063876
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5 Lane 2: RT4 Lane 3: EFO-21 Lane 4: A-431 Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
83
83
Antibody dilution
1:500
1:160
PROTEIN ARRAY
Antibody HPA000992
Antibody HPA063876
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
