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ANTIBODY INFORMATION
 
 

Antibody HPA070307

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA070307  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 16  

References

  

Proper citation

 Atlas Antibodies Cat#HPA070307, RRID:AB_2686249  

VALIDATION SUMMARY
 
 

Antibody HPA070307

  
Immunocytochemistry

Approved  
Western blot

Uncertain  
Protein array

Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA070307

  

Standard validation

 Approved  

 

Figure description

 Immunofluorescent staining of human cell line MCF7 shows localization to endoplasmic reticulum & vesicles.  

Antibody dilution

 1:8  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.  

WESTERN BLOT
 
 

Antibody HPA070307

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

 

Figure description

  

Target mass (kDa)

 32.2  

Antibody dilution

 1:80  

PROTEIN ARRAY
 
 

Antibody HPA070307

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:2000  

ANTIGEN INFORMATION
 
 

Antibody HPA070307

  

Antigen

 Recombinant protein fragment  

Length (aa)

 68  

Antigen sequence

 
HIVADATSSRSMMDRMFALERLARTGIIVTTSEAVLLQLVADKDHPKFKE
IQNLIKASAPESGLLSKV
 

Matching transcripts

 ISOC1-001 - ENSP00000173527 [100%]
 

Other gene match

  
ANTIGEN VIEW
ISOC1-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.