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Immunohistochemical staining of human cerebellum shows strong positivity in the granular layer.
Immunohistochemical staining of human cerebellum shows moderate positivity in neuropil.
Expression
RNA: detected in 22 tissues Protein: detected in 25 cell types
RNA: detected in 22 tissues Protein: detected in 1 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:90
1:200
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA008394
Antibody HPA064788
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY417170)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY417170)
Target mass (kDa)
47.6, 47.3, 19.9, 19
47.6, 47.3, 19.9, 19, 10.2, 7.7, 7.6, 6.2
Antibody dilution
1:250
1:125
PROTEIN ARRAY
Antibody HPA008394
Antibody HPA064788
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.