ACSL4 - Antibodies - The Human Protein Atlas

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ACSL4

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA000286

Antibody HPA005552

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA000286

HPA005552

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA000286, RRID:AB_2665966

Atlas Antibodies Cat#HPA005552, RRID:AB_1078774

VALIDATION SUMMARY

Antibody HPA000286

Antibody HPA005552

Immunocytochemistry

Approved

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA000286

Antibody HPA005552

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.

Antibody dilution

1:50

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA000286

Antibody HPA005552

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 57 cell types

Retrieval

HIER pH6

Antibody dilution

1:100

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA000286

Antibody HPA005552

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

79.2, 74.4

Antibody dilution

1:500

1:250

PROTEIN ARRAY

Antibody HPA000286

Antibody HPA005552

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:2000

1:1000

ANTIGEN INFORMATION

Antibody HPA000286

Antibody HPA005552

Antigen

Recombinant protein fragment

Length (aa)

119

150

Antigen sequence

EAVVHGLNESEASYLITSVELLESKLKTALLDISCVKHIIYVDNKAINKA
EYPEGFEIHSMQSVEELGSNPENLGIPPSRPTPSDMAIVMYTSGSTGRPK
GVMMHHSNLIAGMTGQCER

MNYLEVNRRVNNFGSGLTALGLKPKNTIAIFCETRAEWMIAAQTCFKYNF
PLVTLYATLGKEAVVHGLNESEASYLITSVELLESKLKTALLDISCVKHI
IYVDNKAINKAEYPEGFEIHSMQSVEELGSNPENLGIPPSRPTPSDMAIV

Matching transcripts

ACSL4-001 - ENSP00000262835 [100%]
ACSL4-003 - ENSP00000419171 [100%]
ACSL4-201 - ENSP00000339787 [100%]

Other gene match

ANTIGEN VIEW

ACSL4-001	ACSL4-003	ACSL4-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.