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Immunofluorescent staining of human cell line A-431 shows localization to cytosol & vesicles.
Antibody dilution
1:42
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA000282
Antibody HPA000615
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human gall bladder shows cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.
Expression
RNA: detected in 37 tissues Protein: detected in 50 cell types
RNA: detected in 37 tissues Protein: detected in 59 cell types
Retrieval
HIER pH9
HIER pH6
Antibody dilution
1:1500
1:100
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA000282
Antibody HPA000615
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5 Lane 2: RT4 Lane 3: U-251 MG Lane 4: A-431 Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402754)
Target mass (kDa)
96, 92.8, 90.9, 35
96, 92.8, 90.9
Antibody dilution
1:500
1:250
PROTEIN ARRAY
Antibody HPA000282
Antibody HPA000615
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
