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Antibody staining overlaps with GFP tagged protein
Figure description
Immunofluorescent staining of transgenic HeLa cells show antibody staining in nucleus, nucleoli, cytoplasm & centrosome and GFP expression in nucleus, cytoplasm & centrosome.
Antibody dilution
1:4
Standard validation
Supported
Figure description
Immunofluorescent staining of human cell line A549 shows localization to nucleus, nucleoli & centrosome.
Antibody dilution
1:4
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA004715
Antibody HPA004716
Standard validation
Approved
Figure description
Immunohistochemical staining of human testis shows nuclear positivity in cells of seminiferus ducts.
Expression
RNA: detected in 37 tissues Protein: detected in 52 cell types
Retrieval
HIER pH6
Antibody dilution
1:35
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA004715
Antibody HPA004716
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
84.1, 80, 77.7, 75
84.1, 80, 77.7, 75, 32.7
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA004715
Antibody HPA004716
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
