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TRPC7

ANTIBODY INFORMATION
 
 

Antibody HPA031126

  

Antibody CAB022592

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Chemicon
  

Product name

 HPA031126   AB9326  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 6   5  

References

    

Proper citation

 Atlas Antibodies Cat#HPA031126, RRID:AB_10603221    

VALIDATION SUMMARY
 
 

Antibody HPA031126

  

Antibody CAB022592

  
Immunocytochemistry

Uncertain    
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA031126

  

Antibody CAB022592

  

Standard validation

 Uncertain    

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to actin filaments & focal adhesion sites.    

Antibody dilution

 1:39    

Literature conformity

 The subcellular location is not consistent with literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA031126

  

Antibody CAB022592

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human heart muscle shows strong cytoplasmic and nuclear positivity in myocytes.   Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.  

Expression

 RNA: detected in 0 tissues
Protein: detected in 8 cell types		   RNA: detected in 0 tissues
Protein: detected in 78 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:200   1:750  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA031126

  

Antibody CAB022592

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 99.6, 93.4, 92.4, 86.3   99.6, 93.4, 92.4, 86.3  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA031126

  

Antibody CAB022592

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA031126

  

Antibody CAB022592

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 34    

Antigen sequence

 
QATGELADLIQQLSEKFGKNLNKDHLRVNKGKDI
  

											 
																				

																		

										
											
Matching transcripts

												
																					TRPC7-001 - ENSP00000426070 [100%] 
TRPC7-003 - ENSP00000330322 [100%] 
TRPC7-005 - ENSP00000367720 [100%] 
TRPC7-006 - ENSP00000424854 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

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