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ASNS

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GENERAL INFORMATION

Gene name

 ASNS

Gene description

 Asparagine synthetase (glutamine-hydrolyzing)

Protein class

 Disease related genes
Enzymes
Potential drug targets
Predicted intracellular proteins

Predicted localization

 Intracellular

Number of transcripts

 13
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

Summary

 Localized to the cytosol.

RNA cell category

 Expressed in all

Protein evidence

 Evidence at protein level

Main location

 Localized to the Cytosol (validated)

Single-cell variation

 Single-cell variation in protein expression observed.

Cell cycle dependency

 Cell cycle dependent expression determined by custom assay

DATA RELIABILITY

Reliability score

 Validated based on 2 antibodies.
HPA004924 , HPA064737
SHOW MORE

ASSAYS
RNA expression
Human cells
Custom data

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all
Organ
Origin
Category
Expression
Alphabetical
Cell lines sorted after organ of phenotypic resemblance.

HUMAN CELLS

Summary

 Localized to the cytosol.

Main location

 Cytosol (validated)

 

 

 
Toggle channels
Low
High
G1
S
G2
M
N/A

Thumbnail

Antibody

Cell line

Location

Single-cell variation

Cell cycle dependent variation
HPA004924 A-431 Cytosol
HPA004924 U-2 OS Cytosol
HPA004924 U-251 MG Cytosol
HPA064737 MCF7 Cytosol Intensity
HPA064737 SK-MEL-30 Cytosol Intensity
HPA064737 U-2 OS Cytosol Intensity Intensity

CUSTOM DATA

 

 

 
Toggle channels

Thumbnail

Antibody

Cell line

Assay

Description
HPA064737 U-2 OS Image Staining of U-2 OS FUCCI cells, to characterize the cell cycle dependency of the protein expression pattern. The FUCCI cells, Fluorescence Ubiquitination Cell Cycle Indicator, are cells tagged with different fluorescent proteins fused to two different cell cycle regulators Cdt1 (expressed in G1 phase) and Geminin (expressed in S and G2 phases) that allows cell cycle monitoring. When both proteins are present, the overlay of the images appear in yellow marking the G1/S transition. ASNS is mostly expressed during Mitosis.

CELL CYCLE

Summary

 Expression has cell cycle dependency according to custom assay

Spatial variation

 Cytosol

GENE INFORMATION

Gene name

 ASNS

Synonyms

Description

 Asparagine synthetase (glutamine-hydrolyzing) (HGNC Symbol)

Entrez gene summary

 The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

Chromosome

 7

Cytoband

 q21.3

Chromosome location (bp)

 97852118 - 97872542

Protein evidence

 Evidence at protein level (all genes)

Ensembl

 ENSG00000070669 (version 83.38)

Entrez gene

 440

UniProt

 P08243 (UniProt - Evidence at protein level)

neXtProt

 NX_P08243

Antibodypedia

 ASNS antibodies

PROTEIN BROWSER
«
ASNS-001
ASNS-002
ASNS-005
ASNS-006
ASNS-007
ASNS-008
ASNS-011
ASNS-014
ASNS-015
ASNS-016
ASNS-017
ASNS-021
ASNS-201
»

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)
ASNS-001
ENSP00000377845
ENST00000394308
P08243 [Direct mapping]
Asparagine synthetase [glutamine-hydrolyzing]
Show all
Enzymes
   ENZYME proteins
   Ligase
Predicted intracellular proteins
Disease related genes
Potential drug targets
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0004066 [asparagine synthase (glutamine-hydrolyzing) activity]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005829 [cytosol]
GO:0006529 [asparagine biosynthetic process]
GO:0006541 [glutamine metabolic process]
GO:0008652 [cellular amino acid biosynthetic process]
GO:0030968 [endoplasmic reticulum unfolded protein response]
GO:0034641 [cellular nitrogen compound metabolic process]
GO:0036499 [PERK-mediated unfolded protein response]
GO:0042149 [cellular response to glucose starvation]
GO:0042803 [protein homodimerization activity]
GO:0043066 [negative regulation of apoptotic process]
GO:0044267 [cellular protein metabolic process]
GO:0044281 [small molecule metabolic process]
GO:0045931 [positive regulation of mitotic cell cycle]
GO:0070981 [L-asparagine biosynthetic process]
Show all
 561 aa
64.4 kDa 		No 0
ASNS-002
ENSP00000414901
ENST00000422745
P08243 [Direct mapping]
Asparagine synthetase [glutamine-hydrolyzing]
Show all
Enzymes
   ENZYME proteins
   Ligase
Predicted intracellular proteins
Disease related genes
Potential drug targets
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0004066 [asparagine synthase (glutamine-hydrolyzing) activity]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005829 [cytosol]
GO:0006529 [asparagine biosynthetic process]
GO:0006541 [glutamine metabolic process]
GO:0008652 [cellular amino acid biosynthetic process]
GO:0030968 [endoplasmic reticulum unfolded protein response]
GO:0034641 [cellular nitrogen compound metabolic process]
GO:0036499 [PERK-mediated unfolded protein response]
GO:0042149 [cellular response to glucose starvation]
GO:0042803 [protein homodimerization activity]
GO:0043066 [negative regulation of apoptotic process]
GO:0044267 [cellular protein metabolic process]
GO:0044281 [small molecule metabolic process]
GO:0045931 [positive regulation of mitotic cell cycle]
GO:0070981 [L-asparagine biosynthetic process]
Show all
 540 aa
62.2 kDa 		No 0
ASNS-005
ENSP00000406994
ENST00000444334
P08243 [Direct mapping]
Asparagine synthetase [glutamine-hydrolyzing]
Show all
Enzymes
   ENZYME proteins
   Ligase
Predicted intracellular proteins
Disease related genes
Potential drug targets
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0004066 [asparagine synthase (glutamine-hydrolyzing) activity]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005829 [cytosol]
GO:0006529 [asparagine biosynthetic process]
GO:0006541 [glutamine metabolic process]
GO:0008652 [cellular amino acid biosynthetic process]
GO:0030968 [endoplasmic reticulum unfolded protein response]
GO:0034641 [cellular nitrogen compound metabolic process]
GO:0036499 [PERK-mediated unfolded protein response]
GO:0042149 [cellular response to glucose starvation]
GO:0042803 [protein homodimerization activity]
GO:0043066 [negative regulation of apoptotic process]
GO:0044267 [cellular protein metabolic process]
GO:0044281 [small molecule metabolic process]
GO:0045931 [positive regulation of mitotic cell cycle]
GO:0070981 [L-asparagine biosynthetic process]
Show all
 540 aa
62.2 kDa 		No 0
ASNS-006
ENSP00000408472
ENST00000455086
P08243 [Direct mapping]
Asparagine synthetase [glutamine-hydrolyzing]
Show all
Enzymes
   ENZYME proteins
   Ligase
Predicted intracellular proteins
Disease related genes
Potential drug targets
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0004066 [asparagine synthase (glutamine-hydrolyzing) activity]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005829 [cytosol]
GO:0006529 [asparagine biosynthetic process]
GO:0006541 [glutamine metabolic process]
GO:0008652 [cellular amino acid biosynthetic process]
GO:0030968 [endoplasmic reticulum unfolded protein response]
GO:0034641 [cellular nitrogen compound metabolic process]
GO:0036499 [PERK-mediated unfolded protein response]
GO:0042149 [cellular response to glucose starvation]
GO:0042803 [protein homodimerization activity]
GO:0043066 [negative regulation of apoptotic process]
GO:0044267 [cellular protein metabolic process]
GO:0044281 [small molecule metabolic process]
GO:0045931 [positive regulation of mitotic cell cycle]
GO:0070981 [L-asparagine biosynthetic process]
Show all
 478 aa
54.8 kDa 		No 0
ASNS-007
ENSP00000377846
ENST00000394309
P08243 [Direct mapping]
Asparagine synthetase [glutamine-hydrolyzing]
Show all
Enzymes
   ENZYME proteins
   Ligase
Predicted intracellular proteins
Disease related genes
Potential drug targets
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0004066 [asparagine synthase (glutamine-hydrolyzing) activity]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005829 [cytosol]
GO:0006529 [asparagine biosynthetic process]
GO:0006541 [glutamine metabolic process]
GO:0008652 [cellular amino acid biosynthetic process]
GO:0030968 [endoplasmic reticulum unfolded protein response]
GO:0034641 [cellular nitrogen compound metabolic process]
GO:0036499 [PERK-mediated unfolded protein response]
GO:0042149 [cellular response to glucose starvation]
GO:0042803 [protein homodimerization activity]
GO:0043066 [negative regulation of apoptotic process]
GO:0044267 [cellular protein metabolic process]
GO:0044281 [small molecule metabolic process]
GO:0045931 [positive regulation of mitotic cell cycle]
GO:0070981 [L-asparagine biosynthetic process]
Show all
 561 aa
64.4 kDa 		No 0
ASNS-008
ENSP00000414379
ENST00000437628
P08243 [Direct mapping]
Asparagine synthetase [glutamine-hydrolyzing]
Show all
Enzymes
   ENZYME proteins
   Ligase
Predicted intracellular proteins
Disease related genes
Potential drug targets
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0004066 [asparagine synthase (glutamine-hydrolyzing) activity]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005829 [cytosol]
GO:0006529 [asparagine biosynthetic process]
GO:0006541 [glutamine metabolic process]
GO:0008652 [cellular amino acid biosynthetic process]
GO:0030968 [endoplasmic reticulum unfolded protein response]
GO:0034641 [cellular nitrogen compound metabolic process]
GO:0036499 [PERK-mediated unfolded protein response]
GO:0042149 [cellular response to glucose starvation]
GO:0042803 [protein homodimerization activity]
GO:0043066 [negative regulation of apoptotic process]
GO:0044267 [cellular protein metabolic process]
GO:0044281 [small molecule metabolic process]
GO:0045931 [positive regulation of mitotic cell cycle]
GO:0070981 [L-asparagine biosynthetic process]
Show all
 478 aa
54.8 kDa 		No 0
ASNS-011
ENSP00000397802
ENST00000451771
C9J605 [Direct mapping]
Asparagine synthetase [glutamine-hydrolyzing]
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
 33 aa
3.6 kDa 		No 0
ASNS-014
ENSP00000408797
ENST00000453600
C9JLN6 [Direct mapping]
Asparagine synthetase [glutamine-hydrolyzing]
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
 63 aa
7.5 kDa 		No 0
ASNS-015
ENSP00000400422
ENST00000442734
C9J057 [Direct mapping]
Asparagine synthetase [glutamine-hydrolyzing]
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
 215 aa
24.4 kDa 		No 0
ASNS-016
ENSP00000413797
ENST00000414884
C9JLA3 [Direct mapping]
Asparagine synthetase [glutamine-hydrolyzing]
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
 28 aa
3 kDa 		No 0
ASNS-017
ENSP00000402350
ENST00000448127
C9JM09 [Direct mapping]
Asparagine synthetase [glutamine-hydrolyzing]
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
 119 aa
13.8 kDa 		No 0
ASNS-021
ENSP00000394242
ENST00000437657
C9JT45 [Direct mapping]
Asparagine synthetase [glutamine-hydrolyzing]
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
 162 aa
18.5 kDa 		No 0
ASNS-201
ENSP00000175506
ENST00000175506
P08243 [Direct mapping]
Asparagine synthetase [glutamine-hydrolyzing]
Show all
Enzymes
   ENZYME proteins
   Ligase
Predicted intracellular proteins
Disease related genes
Potential drug targets
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001889 [liver development]
GO:0004066 [asparagine synthase (glutamine-hydrolyzing) activity]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005829 [cytosol]
GO:0006520 [cellular amino acid metabolic process]
GO:0006529 [asparagine biosynthetic process]
GO:0006541 [glutamine metabolic process]
GO:0008652 [cellular amino acid biosynthetic process]
GO:0009416 [response to light stimulus]
GO:0009612 [response to mechanical stimulus]
GO:0009636 [response to toxic substance]
GO:0030968 [endoplasmic reticulum unfolded protein response]
GO:0031427 [response to methotrexate]
GO:0031667 [response to nutrient levels]
GO:0032354 [response to follicle-stimulating hormone]
GO:0032870 [cellular response to hormone stimulus]
GO:0034641 [cellular nitrogen compound metabolic process]
GO:0036499 [PERK-mediated unfolded protein response]
GO:0042149 [cellular response to glucose starvation]
GO:0042803 [protein homodimerization activity]
GO:0043066 [negative regulation of apoptotic process]
GO:0043200 [response to amino acid]
GO:0044267 [cellular protein metabolic process]
GO:0044281 [small molecule metabolic process]
GO:0045931 [positive regulation of mitotic cell cycle]
GO:0048037 [cofactor binding]
GO:0070981 [L-asparagine biosynthetic process]
Show all
 561 aa
64.4 kDa 		No 0

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.