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RAD18

GENERAL INFORMATION

Gene name

 RAD18

Gene description

 RAD18 E3 ubiquitin protein ligase

Protein class

 Plasma proteins
Predicted intracellular proteins

Predicted localization

 Intracellular

Number of transcripts

 6
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HUMAN PROTEIN ATLAS INFORMATION

RNA category
TCGA (cancer tissue):Expressed in all
HPA (cell line):Expressed in all
HPA (normal tissue):Mixed

Protein evidence

 Evidence at protein level

Protein expression
normal tissue

 Selective nuclear expression in selected cell types including lymphoid germinal center cells, basal cells in testis and in the intestine.

ANTIBODY IHC RELIABILITY

Data reliability
description

 Antibody staining mainly consistent with RNA expression data.

Reliability score

 Supported based on 2 antibodies.
HPA006716 , HPA008752
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PROGNOSTIC SUMMARY
Prognostic marker in renal cancer (unfavourable), liver cancer (unfavourable) and prostate cancer (unfavourable).
Renal cancer p<0.001
Liver cancer p<0.001
Prostate cancer p<0.001
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RNA EXPRESSION OVERVIEW
TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION
Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY
HPA006716
HPA008752

GENE INFORMATION

Gene name

 RAD18 (HGNC Symbol)

Synonyms

 RNF73

Description

 RAD18 E3 ubiquitin protein ligase (HGNC Symbol)

Entrez gene summary

 The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq, Jul 2008]

Chromosome

 3

Cytoband

 p25.3

Chromosome location (bp)

 8775402 - 8963773

Protein evidence

 Evidence at protein level (all genes)

Ensembl

 ENSG00000070950 (version 83.38)

Entrez gene

 56852

UniProt

 Q9NS91 (UniProt - Evidence at protein level)

neXtProt

 NX_Q9NS91

Antibodypedia

 RAD18 antibodies

PROTEIN BROWSER
RAD18-001
RAD18-003
RAD18-007
RAD18-008
RAD18-009
RAD18-010

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)
RAD18-001
ENSP00000264926
ENST00000264926
Q9NS91 [Direct mapping]
E3 ubiquitin-protein ligase RAD18
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Predicted intracellular proteins
Plasma proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
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GO:0000403 [Y-form DNA binding]
GO:0000785 [chromatin]
GO:0001741 [XY body]
GO:0003677 [DNA binding]
GO:0003684 [damaged DNA binding]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005657 [replication fork]
GO:0005813 [centrosome]
GO:0006281 [DNA repair]
GO:0006301 [postreplication repair]
GO:0006513 [protein monoubiquitination]
GO:0006974 [cellular response to DNA damage stimulus]
GO:0007283 [spermatogenesis]
GO:0008270 [zinc ion binding]
GO:0009411 [response to UV]
GO:0016874 [ligase activity]
GO:0019985 [translesion synthesis]
GO:0031593 [polyubiquitin binding]
GO:0031625 [ubiquitin protein ligase binding]
GO:0032403 [protein complex binding]
GO:0035861 [site of double-strand break]
GO:0042405 [nuclear inclusion body]
GO:0042769 [DNA damage response, detection of DNA damage]
GO:0043142 [single-stranded DNA-dependent ATPase activity]
GO:0045910 [negative regulation of DNA recombination]
GO:0046872 [metal ion binding]
GO:0051984 [positive regulation of chromosome segregation]
GO:0060548 [negative regulation of cell death]
GO:0061630 [ubiquitin protein ligase activity]
GO:0097505 [Rad6-Rad18 complex]
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 495 aa
56.2 kDa 		No 0
RAD18-003
ENSP00000393783
ENST00000429790
H7C0A5 [Direct mapping]
E3 ubiquitin-protein ligase RAD18
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Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
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GO:0005634 [nucleus]
GO:0006301 [postreplication repair]
GO:0006513 [protein monoubiquitination]
GO:0009411 [response to UV]
GO:0043142 [single-stranded DNA-dependent ATPase activity]
GO:0061630 [ubiquitin protein ligase activity]
GO:0097505 [Rad6-Rad18 complex]
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 79 aa
8.8 kDa 		No 0
RAD18-007
ENSP00000412261
ENST00000413832
C9J0Q4 [Direct mapping]
E3 ubiquitin-protein ligase RAD18
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Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
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GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0006301 [postreplication repair]
GO:0006513 [protein monoubiquitination]
GO:0008270 [zinc ion binding]
GO:0009411 [response to UV]
GO:0043142 [single-stranded DNA-dependent ATPase activity]
GO:0046872 [metal ion binding]
GO:0061630 [ubiquitin protein ligase activity]
GO:0097505 [Rad6-Rad18 complex]
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 92 aa
10.6 kDa 		No 0
RAD18-008
ENSP00000412054
ENST00000427329
H7C3I2 [Direct mapping]
E3 ubiquitin-protein ligase RAD18
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Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
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GO:0005634 [nucleus]
GO:0006301 [postreplication repair]
GO:0006513 [protein monoubiquitination]
GO:0009411 [response to UV]
GO:0043142 [single-stranded DNA-dependent ATPase activity]
GO:0061630 [ubiquitin protein ligase activity]
GO:0097505 [Rad6-Rad18 complex]
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 131 aa
14.7 kDa 		No 0
RAD18-009
ENSP00000399620
ENST00000421052
F8WFA6 [Direct mapping]
E3 ubiquitin-protein ligase RAD18
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Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
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GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0006301 [postreplication repair]
GO:0006513 [protein monoubiquitination]
GO:0008270 [zinc ion binding]
GO:0009411 [response to UV]
GO:0043142 [single-stranded DNA-dependent ATPase activity]
GO:0046872 [metal ion binding]
GO:0061630 [ubiquitin protein ligase activity]
GO:0097505 [Rad6-Rad18 complex]
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 126 aa
14.2 kDa 		No 0
RAD18-010
ENSP00000397852
ENST00000418463
F8WAZ7 [Direct mapping]
E3 ubiquitin-protein ligase RAD18
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Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005634 [nucleus]
GO:0006301 [postreplication repair]
GO:0006513 [protein monoubiquitination]
GO:0009411 [response to UV]
GO:0043142 [single-stranded DNA-dependent ATPase activity]
GO:0061630 [ubiquitin protein ligase activity]
GO:0097505 [Rad6-Rad18 complex]
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 53 aa
6 kDa 		No 0

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.