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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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NCK2
  • TISSUE

  • CELL

  • PATHOLOGY

PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

NCK2

Gene description

NCK adaptor protein 2

Protein class

Plasma proteins
Predicted intracellular proteins

Predicted localization

Intracellular

Number of transcripts

6
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Cell line enhanced (SK-MEL-30)
HPA (normal tissue):Expressed in all

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Cytoplasmic and membranous expression in several tissues.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly consistent with RNA expression data.

Reliability score

Approved based on 2 antibodies.
HPA031004 , CAB016116
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PROGNOSTIC SUMMARY

Gene product is not prognostic.
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Melanoma

STAINING SUMMARY

HPA031004
CAB016116

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Many cases of melanomas showed strong cytoplasmic immunoreactivity. Several malignant carcinoids, prostate and ovarian cancers displayed weak to moderate positivity. Remaining cancer tissues were generally negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

A majority of cancers were weakly stained or negative. Moderate to strong positivity was observed in lymphomas. Prostate cancers showed distinctly stained tumor stroma.

GENE INFORMATION

Gene name

NCK2 (HGNC Symbol)

Synonyms

NCKbeta

Description

NCK adaptor protein 2 (HGNC Symbol)

Entrez gene summary

This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Chromosome

2

Cytoband

q12.2

Chromosome location (bp)

105744897 - 105894274

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000071051 (version 83.38)

Entrez gene

8440

UniProt

O43639 (UniProt - Evidence at protein level)

neXtProt

NX_O43639

Antibodypedia

NCK2 antibodies

PROTEIN BROWSER

NCK2-001
NCK2-002
NCK2-003
NCK2-004
NCK2-005
NCK2-201

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

NCK2-001
ENSP00000233154
ENST00000233154
O43639 [Direct mapping]
Cytoplasmic protein NCK2
Show all
Predicted intracellular proteins
Plasma proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001771 [immunological synapse formation]
GO:0005070 [SH3/SH2 adaptor activity]
GO:0005515 [protein binding]
GO:0005737 [cytoplasm]
GO:0005783 [endoplasmic reticulum]
GO:0005829 [cytosol]
GO:0007015 [actin filament organization]
GO:0007165 [signal transduction]
GO:0007172 [signal complex assembly]
GO:0007173 [epidermal growth factor receptor signaling pathway]
GO:0007176 [regulation of epidermal growth factor-activated receptor activity]
GO:0007411 [axon guidance]
GO:0008093 [cytoskeletal adaptor activity]
GO:0008285 [negative regulation of cell proliferation]
GO:0012506 [vesicle membrane]
GO:0016477 [cell migration]
GO:0030032 [lamellipodium assembly]
GO:0030159 [receptor signaling complex scaffold activity]
GO:0030838 [positive regulation of actin filament polymerization]
GO:0032403 [protein complex binding]
GO:0033137 [negative regulation of peptidyl-serine phosphorylation]
GO:0036493 [positive regulation of translation in response to endoplasmic reticulum stress]
GO:0042102 [positive regulation of T cell proliferation]
GO:0042110 [T cell activation]
GO:0045202 [synapse]
GO:0045944 [positive regulation of transcription from RNA polymerase II promoter]
GO:0048010 [vascular endothelial growth factor receptor signaling pathway]
GO:0048013 [ephrin receptor signaling pathway]
GO:0060996 [dendritic spine development]
GO:0097110 [scaffold protein binding]
GO:0097481 [neuronal postsynaptic density]
GO:1902237 [positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway]
GO:1903898 [negative regulation of PERK-mediated unfolded protein response]
GO:1903912 [negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation]
GO:1990441 [negative regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress]
Show all
380 aa
42.9 kDa
No 0
NCK2-002
ENSP00000377018
ENST00000393349
O43639 [Direct mapping]
Cytoplasmic protein NCK2
Show all
Predicted intracellular proteins
Plasma proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005070 [SH3/SH2 adaptor activity]
GO:0005515 [protein binding]
GO:0005737 [cytoplasm]
GO:0005783 [endoplasmic reticulum]
GO:0005829 [cytosol]
GO:0007165 [signal transduction]
GO:0007172 [signal complex assembly]
GO:0007173 [epidermal growth factor receptor signaling pathway]
GO:0007176 [regulation of epidermal growth factor-activated receptor activity]
GO:0007411 [axon guidance]
GO:0008093 [cytoskeletal adaptor activity]
GO:0008285 [negative regulation of cell proliferation]
GO:0030159 [receptor signaling complex scaffold activity]
GO:0030838 [positive regulation of actin filament polymerization]
GO:0033137 [negative regulation of peptidyl-serine phosphorylation]
GO:0042102 [positive regulation of T cell proliferation]
GO:0042110 [T cell activation]
GO:0045944 [positive regulation of transcription from RNA polymerase II promoter]
GO:0048010 [vascular endothelial growth factor receptor signaling pathway]
GO:0048013 [ephrin receptor signaling pathway]
GO:1903912 [negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation]
Show all
380 aa
42.9 kDa
No 0
NCK2-003
ENSP00000408040
ENST00000425756
E9PEE2 [Direct mapping]
Cytoplasmic protein NCK2
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005515 [protein binding]
Show all
110 aa
12.6 kDa
No 0
NCK2-004
ENSP00000377017
ENST00000393348
E7EPF5 [Direct mapping]
Cytoplasmic protein NCK2
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005515 [protein binding]
Show all
144 aa
16.4 kDa
No 0
NCK2-005
ENSP00000431109
ENST00000522586
E7ERP6 [Direct mapping]
Cytoplasmic protein NCK2
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005515 [protein binding]
Show all
83 aa
9.8 kDa
No 0
NCK2-201
ENSP00000410428
ENST00000451463
E7ERP6 [Direct mapping]
Cytoplasmic protein NCK2
Show all
Predicted intracellular proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005515 [protein binding]
Show all
83 aa
9.8 kDa
No 0

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