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HMMR

ANTIBODY INFORMATION
 
 

Antibody HPA040025

  

Antibody HPA043926

  

Antibody HPA061524

  

Antibody CAB002433

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Novocastra
  

Product name

 HPA040025   HPA043926   HPA061524   NCL-CD168  

Host species

 Rabbit   Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

        

Released in version

 13   12   16   1  

References

        

Proper citation

 Atlas Antibodies Cat#HPA040025, RRID:AB_2676805     Atlas Antibodies Cat#HPA061524, RRID:AB_2684551    

VALIDATION SUMMARY
 
 

Antibody HPA040025

  

Antibody HPA043926

  

Antibody HPA061524

  

Antibody CAB002433

  
Immunocytochemistry

 

Validated
  

Validated
   Approved  
Immunohistochemistry

Approved      

Validated
  
Western blot

Supported   Uncertain   Uncertain   Supported  
Protein array

Approved   Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA040025

  

Antibody HPA043926

  

Antibody HPA061524

  

Antibody CAB002433

  

Formal validation: Independent

  
Validated

Antibody staining overlaps with antibody HPA061524.		  
Validated

Antibody staining overlaps with antibody HPA043926.		    
 

Standard validation

   Approved   Approved   Approved  

       

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to centrosome & microtubules.   Immunofluorescent staining of human cell line SiHa shows localization to microtubules.   Immunofluorescent staining of human cell line U-251 MG shows localization to microtubules.  

Antibody dilution

   1:52   1:200   1:50  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA040025

  

Antibody HPA043926

  

Antibody HPA061524

  

Antibody CAB002433

  

Formal validation: Orthogonal

      
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

     
 

Figure description

       Distribution of protein expression (antibody staining) and RNA expression (TPM) across 36 tissues.  
 

Standard validation

 Approved       Approved  

       

Figure description

 Immunohistochemical staining of human testis shows strong cytoplasmic positivity in a subset of cells in seminiferous ducts.       Immunohistochemical staining of human testis shows strong cytoplasmic positivity in a subset of cells in seminiferous ducts.  

Expression

 RNA: detected in 23 tissues
Protein: detected in 19 cell types		       RNA: detected in 23 tissues
Protein: detected in 27 cell types		  

Retrieval

 HIER pH6       HIER pH9  

Antibody dilution

 1:4000       1:50  

Literature conformity

 Partly consistent with gene/protein characterization data.       Partly consistent with gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.       Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA040025

  

Antibody HPA043926

  

Antibody HPA061524

  

Antibody CAB002433

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

       

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 84.2, 84.1, 82.3, 74.5   84.2, 84.1, 82.3   84.2, 84.1, 82.3, 74.5   84.2, 84.1, 82.3, 74.5, 11.7, 10.7  

Antibody dilution

 1:250   1:250   1:1080   1:500  

PROTEIN ARRAY
 
 

Antibody HPA040025

  

Antibody HPA043926

  

Antibody HPA061524

  

Antibody CAB002433

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:12000   1:3000   1:12950    

ANTIGEN INFORMATION
 
 

Antibody HPA040025

  

Antibody HPA043926

  

Antibody HPA061524

  

Antibody CAB002433

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 95   94   89    

Antigen sequence

 
SSAAHTQATLLLQEKYDSMVQSLEDVTAQFESYKALTASEIEDLKLENSS
LQEKVAKAGKNAEDVQHQILATESSNQEYVRMLLDLQTKSALKET
  
KRFNDPSGCAPSPGAYDVKTLEVLKGPVSFQKSQRFKQQKESKQNLNVDK
DTTLPASARKVKSSESKKESQKNDKDLKILEKEIRVLLQERGAQ
  
LKQQEEDFRKQLEDEEGRKAEKENTTAELTEEINKWRLLYEELYNKTKPF
QLQLDAFEVEKQALLNEHGAAQEQLNKIRDSYAKLLGHQ
   

Matching transcripts

 HMMR-001 - ENSP00000377492 [99%]
HMMR-002 - ENSP00000351554 [99%]
HMMR-003 - ENSP00000185942 [99%]
HMMR-006 - ENSP00000402673 [99%]
  HMMR-001 - ENSP00000377492 [100%]
HMMR-002 - ENSP00000351554 [99%]
HMMR-003 - ENSP00000185942 [83%]
  HMMR-001 - ENSP00000377492 [100%]
HMMR-002 - ENSP00000351554 [100%]
HMMR-003 - ENSP00000185942 [100%]
HMMR-006 - ENSP00000402673 [100%]
   

Other gene match

        
ANTIGEN VIEW
HMMR-001
HMMR-002
HMMR-003
HMMR-006
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.