P4HA2 - Antibodies - The Human Protein Atlas

We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.

P4HA2

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA016997

Antibody HPA027824

Antibody CAB062557

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA016997

HPA027824

AMAb90710

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA016997, RRID:AB_2669501

Atlas Antibodies Cat#HPA027824, RRID:AB_2672402

Atlas Antibodies Cat#AMAb90710, RRID:AB_2665641

VALIDATION SUMMARY

Antibody HPA016997

Antibody HPA027824

Antibody CAB062557

Immunocytochemistry

Supported

Immunohistochemistry

Western blot

Supported

Protein array

Approved

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA016997

Antibody HPA027824

Antibody CAB062557

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum & vesicles.

Antibody dilution

1:57

1:50

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA016997

Antibody HPA027824

Antibody CAB062557

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA027824 across 73 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA016997 across 73 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human uterus shows strong cytoplasmic positivity in glandular cells.

Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in decidual cells.

Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 55 cell types

RNA: detected in 37 tissues
Protein: detected in 41 cell types

RNA: detected in 37 tissues
Protein: detected in 53 cell types

Retrieval

HIER pH6

Antibody dilution

1:400

1:250

Literature conformity

Consistent with extensive gene/protein characterization data.

Partly consistent with extensive gene/protein characterization data.

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA016997

Antibody HPA027824

Antibody CAB062557

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

60.9, 60.6, 26.6, 22.9, 20.6, 18.7, 17.1, 16.5

60.9, 60.6

60.9, 60.6, 26.6, 22.9, 20.6, 18.7, 17.1, 16.5, 12.7, 11.4, 5.8

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA016997

Antibody HPA027824

Antibody CAB062557

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA016997

Antibody HPA027824

Antibody CAB062557

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

129

137

Antigen sequence

LKEYILVEEAKLSKIKSWANKMEALTSKSAADAEGYLAHPVNAYKLVKRL
NTDWPALEDLVLQDSAAGFIANLSVQRQFFPTDEDEIGAAKALMRLQDTY
RLDPGTISRGELPGTKYQAMLSVDDCFGM

LDYLSYAVFQLGDLHRALELTRRLLSLDPSHERAGGNLRYFEQLLEEERE
KTLTNQTEAELATPEGIYERPVDYLPERDVYESLCRGEGVKLTPRRQKRL
FCRYHHGNRAPQLLIAPFKEEDEWDSPHIVRYYDVMS

Matching transcripts

P4HA2-001 - ENSP00000384999 [100%]
P4HA2-002 - ENSP00000353772 [100%]
P4HA2-003 - ENSP00000166534 [100%]
P4HA2-004 - ENSP00000368379 [100%]
P4HA2-010 - ENSP00000389523 [100%]
P4HA2-014 - ENSP00000391257 [100%]
P4HA2-015 - ENSP00000405406 [100%]
P4HA2-201 - ENSP00000368394 [100%]
P4HA2-202 - ENSP00000368398 [100%]
P4HA2-017 - ENSP00000378593 [96%]
P4HA2-005 - ENSP00000413542 [85%]
P4HA2-009 - ENSP00000396495 [81%]

P4HA2-001 - ENSP00000384999 [100%]
P4HA2-002 - ENSP00000353772 [100%]
P4HA2-003 - ENSP00000166534 [100%]
P4HA2-004 - ENSP00000368379 [100%]
P4HA2-201 - ENSP00000368394 [100%]
P4HA2-202 - ENSP00000368398 [100%]

Other gene match

ANTIGEN VIEW

P4HA2-001	P4HA2-002	P4HA2-003	P4HA2-004	P4HA2-005	P4HA2-009	P4HA2-010	P4HA2-014	P4HA2-015	P4HA2-017	P4HA2-201	P4HA2-202

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.